Literature summary for 5.6.1.1 extracted from

White, S.R.; Evans, K.J.; Lary, J.; Cole, J.L.; Lauring, B.
Recognition of C-terminal amino acids in tubulin by pore loops in Spastin is important for microtubule severing (2007), J. Cell Biol., 176, 995-1005.

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Application

Application	Comment	Organism
medicine	spastin is mutated in the neurodegenerative disease hereditary spastic paraplegia	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
into the pGEX-6P-3 vector for expression in Escherichia coli BL21-CodonPlus DE3-RIPL cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
1-279STOP	deletion mutant, no severing of microtubules, no binding of microtubules	Homo sapiens
1-328STOP	deletion mutant, no severing of microtubules, but binding of microtubules	Homo sapiens
DELTA1-227	deletion mutant, severs microtubules, binds microtubules	Homo sapiens
DELTAMTBD	deletion mutant lacking aa 1-227 and 270-328, neither binds nor severs microtubules	Homo sapiens
E442Q	mutant harboring a canonical Walker B motif point mutation, predicted to bind but not hydrolyze ATP	Homo sapiens
fragment 227-279	fragment consisting of aa 227-279, no severing of microtubules, no binding of microtubules	Homo sapiens
fragment 227-328	fragment consisting of aa 227-328, no severing of microtubules, but binding of microtubules	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.45	-	ATP	full-length spastin	Homo sapiens
2.2	-	ATP	mutant DELTA1-227	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	full-length spastin without tag, determined by SDS-PAGE	Homo sapiens
69000	-	GST-spastin, determined by SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O	Homo sapiens	-	ADP + phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using a glutathione-Sepharose column, cleavage of the GST moiety with PreScission Protease	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Homo sapiens	ADP + phosphate	-	ir

Subunits

Subunits	Comment	Organism
hexamer	assembles into a hexamer in the presence of ATP	Homo sapiens

Synonyms

Synonyms	Comment	Organism
microtubule-severing ATPase	-	Homo sapiens
spastin	-	Homo sapiens

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Release 2023.1 (January 2023)