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Literature summary for 5.6.1.1 extracted from
- Human spastin has multiple microtubule-related functions (2005), J. Neurochem., 95, 1411-1420.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| microtubule | - |
Homo sapiens |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the most frequently mutated gene causing autosomal dominant hereditary spastic paraplegias is SPG4, which encodes spastin | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pQE30 vector, His-tag, and pGEX 4T3, GST-fusion, for expression in Escherichia coli | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54400 | - |
spastin isoform: splice variant lacking exon 4, 2nd ATG, predicted molecular weight of the isoform tagged with His6 | Homo sapiens |
| 58000 | - |
spastin isoform: 2nd ATG, predicted molecular weight of the isoform tagged with His6 | Homo sapiens |
| 62200 | - |
spastin isoform: 1st ATG, predicted molecular weight of the isoform tagged with His6 | Homo sapiens |
| 63600 | - |
spastin isoform: splice variant lacking exon 4, 1st ATG, predicted molecular weight of the isoform tagged with His6 | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9UBP0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column, glutathione-agarose beads | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEK293 cell | - |
Homo sapiens | - |
| HeLa cell | - |
Homo sapiens | - |
| SH-SY5Y cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000045 | - |
His6-spastin | Homo sapiens |
| 0.000046 | - |
GST-spastin | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| spastin | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
ATPase assay | Homo sapiens |
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Release 2023.1 (January 2023)
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