Literature summary for 5.6.1.1 extracted from

Evans, K.J.; Gomes, E.R.; Reisenweber, S.M.; Gundersen, G.G.; Lauring, B.P.
Linking axonal degeneration to microtubule remodeling by Spastin-mediated microtubule severing (2005), J. Cell Biol., 168, 599-606.

Search for more literature for 5.6.1.1

Application

Application	Comment	Organism
medicine	mutations cause an autosomal dominant form of hereditary spastic paraplegia	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in NIH-3T3 cells	Homo sapiens
spastin cDNA was cloned into pGEX6p-3 for production of recombinant protein in Escherichia coli or into pEYFP-C1	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E442Q	mutant, no significant enzymatic activity	Homo sapiens
K388A	mutant, no significant enzymatic activity	Homo sapiens
K388R	disease-associated mutation, no significant enzymatic activity	Homo sapiens
N386K	disease-associated mutation, no significant enzymatic activity	Homo sapiens
R499C	disease-associated mutation, no significant enzymatic activity	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.27	-	ATP	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microtubule	-	Homo sapiens	5874	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
95000	-	GST-spastin analyzed on a 12%-PAG	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O	Homo sapiens	-	ADP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification of recombinant protein using a glutathione-sepharose column, removing of the GST moiety with precision protease	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Homo sapiens	ADP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
spastin	-	Homo sapiens
SPG4	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	ATPase acitvity assay	Homo sapiens
37	-	microtubule-binding assay	Homo sapiens
37	-	microtubule-severing assay	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)