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Literature summary for 5.6.1.1 extracted from

  • Roll-Mecak, A.; Vale, R.D.
    The Drosophila homologue of the hereditary spastic paraplegia protein, spastin, severs and disassembles microtubules (2005), Curr. Biol., 15, 650-655.
    View publication on PubMed
Search for more literature for 5.6.1.1

Application

Application Comment Organism
medicine hereditary spastic paraplegias (HSPs) are most commonly caused by mutations in the spastin gene Drosophila melanogaster

Cloned(Commentary)

Cloned (Comment) Organism
D-spastin-GFP for localization studies Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
D655N disease mutation Drosophila melanogaster
E542A disease mutation, Walker B motif Drosophila melanogaster
K488R disease mutation, Walker A motif Drosophila melanogaster
additional information deltaTM D-spastin-GFP: construct lacking the transmembrane-containing N-terminus, diffusely distributed in the cytoplasm, fully active for disassembling cytoplasmatic microtubules. TM+MIT: construct lacking the AAA domain, do not affect the microtubule cytoskeleton. MIT: MIT domain alone, diffusely distributed and do not disassemble microtubules. AAA: AAA domain alone, diffusely distributed and do not disassemble microtubules. Drosophila melanogaster
S462C disease mutation Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Drosophila melanogaster 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Drosophila melanogaster
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
S2 cell
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Drosophila melanogaster ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
D-spastin Drosophila homolog of spastin Drosophila melanogaster
spastin
-
 Drosophila melanogaster

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
 ATP mutant D655N Drosophila melanogaster
1.1
-
 ATP mutant S462C Drosophila melanogaster
1.8
-
 ATP wild type, deltaTM Drosophila melanogaster