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Literature summary for 5.5.1.8 extracted from

  • Croteau, R.; Karp, F.
    Biosynthesis of monoterpenes: preliminary characterization of bornyl pyrophosphate synthetase from sage (Salvia officinalis) and demonstration that geranyl pyrophosphate is the preferred substrate for cyclization (1979), Arch. Biochem. Biophys., 198, 512-522.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate
-
Salvia officinalis
p-hydroxymercuribenzoate
-
Salvia officinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
geranyl diphosphate
-
Salvia officinalis
0.018
-
neryl diphosphate
-
Salvia officinalis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Salvia officinalis
Mg2+ Km: 3.5 mM Salvia officinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
gel filtration Salvia officinalis

Organism

Organism UniProt Comment Textmining
Salvia officinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Salvia officinalis

Reaction

Reaction Comment Organism Reaction ID
geranyl diphosphate = (+)-bornyl diphosphate mechanism Salvia officinalis

Source Tissue

Source Tissue Comment Organism Textmining
leaf unfurled leaves from the shoot apex Salvia officinalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Geranyl diphosphate geranyl diphosphate is preferred over neryl diphosphate Salvia officinalis (+)-Bornyl-diphosphate
-
?
Neryl diphosphate geranyl diphosphate is preferred over neryl diphosphate Salvia officinalis (+)-Bornyl-diphosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
geranyl diphosphate and neryl diphosphate, the maximal activity with geranyl diphosphate is about 5times higher than with neryl diphosphate Salvia officinalis

pH Range

pH Minimum pH Maximum Comment Organism
5.7 6.7 pH 5.7: about 40% of maximal activity, pH 6.7: about 35% of maximal activity, geranyl diphosphate Salvia officinalis