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Literature summary for 5.5.1.7 extracted from
- Metabolism of aniline and 3-chloroaniline in Pseudomonas acidovorans CA28: evidence of isofunctional muconate cycloisomerases (1993), J. Basic Microbiol., 33, 301-309.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
3-chloro-cis,cis-muconate | - |
Delftia acidovorans |  |
| 0.065 | - |
2-methyl-cis,cis-muconate | - |
Delftia acidovorans | |
| 0.083 | - |
cis,cis-muconate | - |
Delftia acidovorans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Delftia acidovorans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Delftia acidovorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.058 | - |
- |
Delftia acidovorans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 24 h, complete loss of activity | Delftia acidovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Methyl-cis,cis-muconate | - |
Delftia acidovorans | ? | - |
? | |
| 2-Methyl-cis,cis-muconate | - |
Delftia acidovorans CA28 | ? | - |
? | |
| 3-Chloro-cis,cis-muconate | about 90% of the activity compared to 2-methyl-cis,cis-muconate | Delftia acidovorans | cis-4-Carboxymethylenebut-2-en-4-olide | - |
? | |
| 3-Chloro-cis,cis-muconate | about 90% of the activity compared to 2-methyl-cis,cis-muconate | Delftia acidovorans CA28 | cis-4-Carboxymethylenebut-2-en-4-olide | - |
? | |
| cis,cis-Muconate | about 30% of the activity compared to 2-methyl-cis,cis-muconate | Delftia acidovorans | ? | - |
? | |
| cis,cis-Muconate | about 30% of the activity compared to 2-methyl-cis,cis-muconate | Delftia acidovorans CA28 | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, strongly effected | Delftia acidovorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Delftia acidovorans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH 6: about 60% of maximal activity, pH 8.5: about 40% of maximal activity | Delftia acidovorans |
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