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Literature summary for 5.5.1.6 extracted from
- First bacterial chalcone isomerase isolated from Eubacterium ramulus (2004), Arch. Microbiol., 181, 428-434.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM, 25% inactivation | Eubacterium ramulus |  |
| N-bromosuccinimide | 1 mM, complete inhibition | Eubacterium ramulus | |
| naringenin | 0.01 mM, 30% inhibition | Eubacterium ramulus | |
| NEM | 10 mM, slight inactivation | Eubacterium ramulus | |
| phloretin | 0.001 mM, 36% inhibition | Eubacterium ramulus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0075 | - |
butein | - |
Eubacterium ramulus | |
| 0.0143 | - |
isoliquiritigenin | - |
Eubacterium ramulus | |
| 0.043 | - |
naringenin chalcone | - |
Eubacterium ramulus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
4 * 30000, SDS-PAGE | Eubacterium ramulus |
| 129000 | - |
non-denaturing PAGE | Eubacterium ramulus |
| 130000 | - |
gel filtration | Eubacterium ramulus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eubacterium ramulus | V9P0A9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Eubacterium ramulus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 914 | - |
- |
Eubacterium ramulus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butein | - |
Eubacterium ramulus | (S)-eriodictyol | - |
? | |
| isoliquiritigenin | - |
Eubacterium ramulus | liquiritigenin | - |
? | |
| naringenin chalcone | - |
Eubacterium ramulus | naringenin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 30000, SDS-PAGE | Eubacterium ramulus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Eubacterium ramulus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 38.33 | - |
isoliquiritigenin | - |
Eubacterium ramulus | |
| 48.33 | - |
butein | - |
Eubacterium ramulus | |
| 2300 | - |
naringenin chalcone | - |
Eubacterium ramulus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 7 | - |
Eubacterium ramulus |
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Release 2023.1 (January 2023)
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