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Literature summary for 5.5.1.6 extracted from
- Purification and properties of chalcone isomerase from cell suspension cultures of Phaseolus vulgaris (1982), Biochim. Biophys. Acta, 715, 25-33.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2',4'-Dihydroxy-4-methoxychalcone | substrate inhibition at 0.025 mM and above | Phaseolus vulgaris |  |
| 2',4'-Dimethoxy-4-hydroxychalcone | inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
| 3,9-dihydroxypterocarpan | inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
| 4,2',4'-trihydroxychalcone | substrate inhibition at 0.025 mM and above | Phaseolus vulgaris | |
| 5,7,2',4'-Tetrahydroxy-8-(3,3-dimethylallyl)isoflavanone | i.e. kievitone, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
| 5,7,2',4'-Tetrahydroxyisoflavanone | i.e. dalbergioidin, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
| 5,7,2',4'-tetrahydroxyisoflavone | i.e. 2'-hydroxygenistein, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
| 5,7,4',5'-Tetrahydroxyflavanol | i.e. quercetin, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
| 5,7,4'-Trihydroxyflavanone | - |
Phaseolus vulgaris | |
| 5,7,4'-trihydroxyflavonol | - |
Phaseolus vulgaris | |
| 5,7,4'-trihydroxyisoflavone | i.e. genistein, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
| 7,12-Dihydroxycoumestan | i.e. coumestrol, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
| 7-Hydroxy-3',4'-dimethylchromanocoumaran | i.e. phaseollin, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
| HgCl2 | - |
Phaseolus vulgaris | |
| PCMB | - |
Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
2',4'-Dihydroxy-4-methoxychalcone | - |
Phaseolus vulgaris | |
| 0.0089 | - |
4,2',4'-trihydroxychalcone | - |
Phaseolus vulgaris | |
| 0.0133 | - |
4,2',4',6'-Tetrahydroxychalcone | - |
Phaseolus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
1 * 28000, SDS-PAGE | Phaseolus vulgaris |
| 30000 | - |
gel filtration | Phaseolus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Phaseolus vulgaris | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 5% glycerol, 35% loss of activity after 6 weeks | Phaseolus vulgaris |
| 4°C, 0.129 mg enzyme per ml, 95% of the original activity is retained after 6 days | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2',4'-Dihydroxy-4-methoxychalcone | - |
Phaseolus vulgaris | ? | - |
? | |
| 4,2',4',6'-Tetrahydroxychalcone | - |
Phaseolus vulgaris | 5,7,4'-Trihydroxyflavanone | - |
? | |
| 4,2',4'-trihydroxychalcone | i.e. isoliquiritigenin | Phaseolus vulgaris | (2S)-4',7-dihydroxyflavanone | the product is liquiritigenin | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28000, SDS-PAGE | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
4,2',4'-trihydroxychalcone | Phaseolus vulgaris |
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