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Literature summary for 5.5.1.4 extracted from

  • Lu, Y.; Wang, L.; Teng, F.; Zhang, J.; Hu, M.; Tao, Y.
    Production of myo-inositol from glucose by a novel trienzymatic cascade of polyphosphate glucokinase, inositol 1-phosphate synthase and inositol monophosphatase (2018), Enzyme Microb. Technol., 112, 1-5 .
    View publication on PubMed
Search for more literature for 5.5.1.4

Application

Application Comment Organism
synthesis production of myo-inositol from glucose by a novel trienzymatic cascade of polyphosphate glucokinase, inositol 1-phosphate synthase and inositol monophosphatase. myo-Inositol (inositol) is important in the cosmetics, pharmaceutical and functional food industries. The conversion ratio from glucose to inositol reaches 90%, which is promising for the enzymatic synthesis of inositol without ATP supplementation Trypanosoma brucei

Cloned(Commentary)

Cloned (Comment) Organism
-
 Trypanosoma brucei
expressed in Escherichia coli Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by up to 50 mM D-glucose Trypanosoma brucei
phosphate the enzyme activity is almost completely abolished at 125 mM Trypanosoma brucei
sodium hexametaphosphate the enzyme activity is almost completely abolished at 20 mM Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Glucose 6-phosphate Trypanosoma brucei
-
 L-myo-Inositol 1-phosphate
-
 ir
D-Glucose 6-phosphate Trypanosoma brucei TREU927
-
 L-myo-Inositol 1-phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-
Trypanosoma brucei Q38AQ2
-
-
Trypanosoma brucei TREU927
-
-
-
Trypanosoma brucei TREU927 Q38AQ2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trypanosoma brucei
Ni-NTA column chromatography Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose 6-phosphate
-
 Trypanosoma brucei L-myo-Inositol 1-phosphate
-
 ir
D-Glucose 6-phosphate
-
 Trypanosoma brucei TREU927 L-myo-Inositol 1-phosphate
-
 ir

Synonyms

Synonyms Comment Organism
Inositol 1-phosphate synthase
-
 Trypanosoma brucei
IPS
-
 Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
NAD+ required Trypanosoma brucei

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
15
-
 at pH 7.0 and 37°C Trypanosoma brucei sodium hexametaphosphate
50
-
 at pH 7.0 and 37°C Trypanosoma brucei phosphate