Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, expression of His-tagged INOS in Escherichia coli strain BL21(DE3) | Drosophila melanogaster |
General Stability | Organism |
---|---|
20% v/v glycerol, 2 mM PMSF, and 1 mM E-64 stabilize the native and the recombinant enzyme | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | dissociation constants | Drosophila melanogaster | |
0.42 | - |
NAD+ | pH 7.5, 37°C, 7.5, recombinant enzyme | Drosophila melanogaster | |
3.5 | - |
D-glucose 6-phosphate | pH 7.5, 37°C, 7.5, recombinant enzyme | Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
4 * 62000, SDS-PAGE | Drosophila melanogaster |
271000 | - |
approximately, recombinant enzyme, gel filtration | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged INOS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Drosophila melanogaster |
Storage Stability | Organism |
---|---|
-20°C, purified recombinant enzyme, 20% v/v glycerol, completely stable for several weeks | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | - |
Drosophila melanogaster | 1D-myo-inositol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 62000, SDS-PAGE | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
iNOS | - |
Drosophila melanogaster |
Myo-inositol-1-phosphate synthase | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | Drosophila melanogaster |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | increasing activity from 20°C to 40 °C, rapid loss of 60% activity at 50°C, inactive at 60°C | Drosophila melanogaster |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
rapid loss of 60% activity | Drosophila melanogaster |
60 | - |
complete loss of activity after 5 min | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
recombinant enzyme | Drosophila melanogaster |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | 34% of maximal activity at pH 9.0, low activity below pH 5.0, inactive above pH 11.0 | Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Drosophila melanogaster |