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Literature summary for 5.5.1.35 extracted from

  • Lichman, B.R.; Kamileen, M.O.; Titchiner, G.R.; Saalbach, G.; Stevenson, C.E.M.; Lawson, D.M.; O'Connor, S.E.
    Uncoupled activation and cyclization in catmint reductive terpenoid biosynthesis (2019), Nat. Chem. Biol., 15, 71-79 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with NAD+. Cofactor is not utilized during catalysis, but appears to be important for the enzyme structure Nepeta racemosa

Protein Variants

Protein Variants Comment Organism
A151T mutation abolishes detectable soluble expression Nepeta racemosa
G152T mutation abolishes detectable soluble expression Nepeta racemosa
K169M severe reduction of activity Nepeta racemosa
L156S reduced dehydrogenase activity Nepeta racemosa
M196S severe reduction of activity Nepeta racemosa
N150T activity similar to wild-type Nepeta racemosa
S153P mutation abolishes detectable soluble expression Nepeta racemosa
S154L no detectable cis-cis cyclase activity, though at higher enzyme concentrations formation of cis,trans-nepetalactol, reaction of EC 5.5.1.34 Nepeta racemosa
Y165F mutation abolishes detectable soluble expression Nepeta racemosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-8-oxocitronellyl enol Nepeta racemosa
-
(+)-cis,cis-nepetalactol
-
?

Organism

Organism UniProt Comment Textmining
Nepeta racemosa A0A4P1LYE8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
trichome glandular trichome Nepeta racemosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-8-oxocitronellyl enol
-
Nepeta racemosa (+)-cis,cis-nepetalactol
-
?

Synonyms

Synonyms Comment Organism
nepetalactol-related SDR 3
-
Nepeta racemosa
NEPS3
-
Nepeta racemosa

Cofactor

Cofactor Comment Organism Structure
NAD+ enzyme binds NAD+. NEPS3 shows no notable oxidation activity with NAD+ or NADP+ or at different pHs Nepeta racemosa

General Information

General Information Comment Organism
physiological function isoforms Neps1 and Neps2 catalyze the stereoselective cyclisation of reactive intermediate (S)-8-oxocitronellyl enol into nepetalactol. Subsequent oxidation of nepetalactol by NEPS1 provides nepetalactone, metabolites known for both insect-repellent activity and euphoric effect in cats Nepeta racemosa