Crystallization (Comment) | Organism |
---|---|
in complex with NAD+. Cofactor is not utilized during catalysis, but appears to be important for the enzyme structure | Nepeta racemosa |
Protein Variants | Comment | Organism |
---|---|---|
A151T | mutation abolishes detectable soluble expression | Nepeta racemosa |
G152T | mutation abolishes detectable soluble expression | Nepeta racemosa |
K169M | severe reduction of activity | Nepeta racemosa |
L156S | reduced dehydrogenase activity | Nepeta racemosa |
M196S | severe reduction of activity | Nepeta racemosa |
N150T | activity similar to wild-type | Nepeta racemosa |
S153P | mutation abolishes detectable soluble expression | Nepeta racemosa |
S154L | no detectable cis-cis cyclase activity, though at higher enzyme concentrations formation of cis,trans-nepetalactol, reaction of EC 5.5.1.34 | Nepeta racemosa |
Y165F | mutation abolishes detectable soluble expression | Nepeta racemosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-8-oxocitronellyl enol | Nepeta racemosa | - |
(+)-cis,cis-nepetalactol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nepeta racemosa | A0A4P1LYE8 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trichome | glandular trichome | Nepeta racemosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-8-oxocitronellyl enol | - |
Nepeta racemosa | (+)-cis,cis-nepetalactol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nepetalactol-related SDR 3 | - |
Nepeta racemosa |
NEPS3 | - |
Nepeta racemosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | enzyme binds NAD+. NEPS3 shows no notable oxidation activity with NAD+ or NADP+ or at different pHs | Nepeta racemosa |
General Information | Comment | Organism |
---|---|---|
physiological function | isoforms Neps1 and Neps2 catalyze the stereoselective cyclisation of reactive intermediate (S)-8-oxocitronellyl enol into nepetalactol. Subsequent oxidation of nepetalactol by NEPS1 provides nepetalactone, metabolites known for both insect-repellent activity and euphoric effect in cats | Nepeta racemosa |