Protein Variants | Comment | Organism |
---|---|---|
D391G/D392G | site-directed mutagenesis of DIDD motif residues, the mutant lacks type-B activity | Selaginella moellendorffii |
D611G/D612G | site-directed mutagenesis of DDLMD motif residues, the mutant lacks type-A cyclase activity | Selaginella moellendorffii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Selaginella moellendorffii | G9MAN7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | BDTC-like enzyme, miltiradiene synthase converts geranylgeranyl diphosphate to a diterpene hydrocarbon product with a molecular mass of 272 Da. Mutation in the type-B active motif of BDTC-like enzyme miltiradiene synthase, D611G/D612G, abolishes the cyclase activity, whereas (+)-copalyl diphosphate, the reaction intermediate from the conversion of geranylgeranyl diphosphate to the hydrocarbon product, rescues the cyclase activity of the mutant to form a diterpene hydrocarbon. Another mutant lacking type-A activity, D391G/D392G, accumulates copalyl diphosphate as the reaction intermediate | Selaginella moellendorffii | (+)-copalyl diphosphate | - |
? | |
additional information | miltiradiene synthesis reaction with geranylgeranyl diphosphate to miltiradiene, EC 4.2.3.131, via (+)-copalyl diphosphate intermediate, not ent-copalyl diphosphate. The enzyme catalyzes the successive two-step type-B (protonation-initiated cyclization) and type-A (ionization-initiated cyclization) reactions of geranylgeranyl diphosphate. The two aspartate-rich motifs, 389DIDD and 611DDLMD, that are required for the type-B and type-A cyclization reactions, respectively. Product analysis by GC-MS and NMR spectroscopy | Selaginella moellendorffii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BDTC | - |
Selaginella moellendorffii |
bifunctional diterpene cyclase | - |
Selaginella moellendorffii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Selaginella moellendorffii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Selaginella moellendorffii |
General Information | Comment | Organism |
---|---|---|
metabolism | bifunctional diterpene cyclases are involved in hormone and defense compound biosyntheses in bryophytes and gymnosperms, respectively | Selaginella moellendorffii |
additional information | enzyme structure by two-dimensional and three-dimensional NMR | Selaginella moellendorffii |