Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Abies grandis |
Protein Variants | Comment | Organism |
---|---|---|
D361A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D402A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D402E | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D402N | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D404A | - |
Abies grandis |
D404E | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D404N | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D405A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D405E | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
D405N | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
E499A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
R365A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
R411A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
R454A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
W358A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
Y520A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
14,15-dihydro-15-aza-geranylgeranyl diphosphate | - |
Abies grandis | |
geranylgeranyl diphosphate | - |
Abies grandis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
geranylgeranyl diphosphate | W358A | Abies grandis | |
0.0006 | - |
geranylgeranyl diphosphate | D405N | Abies grandis | |
0.0008 | - |
geranylgeranyl diphosphate | D361A | Abies grandis | |
0.001 | - |
geranylgeranyl diphosphate | D402A, D402E, D405E, R454A | Abies grandis | |
0.002 | - |
geranylgeranyl diphosphate | E499A, R411A, R365A | Abies grandis | |
0.003 | - |
geranylgeranyl diphosphate | wild-type, D405A, Y520A | Abies grandis | |
0.007 | - |
geranylgeranyl diphosphate | D404A | Abies grandis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM selectively enabled the conversion of copalyl diphosphate | Abies grandis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | Abies grandis | - |
(+)-copalyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abies grandis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Abies grandis | (+)-copalyl diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
geranylgeranyl diphosphate | D405N, Y520A | Abies grandis | |
0.2 | - |
geranylgeranyl diphosphate | D402N, R365A, R454A | Abies grandis | |
0.5 | - |
geranylgeranyl diphosphate | R411A | Abies grandis | |
2.2 | - |
geranylgeranyl diphosphate | - |
Abies grandis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
with geranylgeranyl diphosphate as substrate | Abies grandis |