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Literature summary for 5.5.1.12 extracted from

  • Peters, R.J.; Croteau, R.B.
    Abietadiene synthase catalysis: conserved residues involved in protonation-initiated cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate (2002), Biochemistry, 41, 1836-1842.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Abies grandis

Protein Variants

Protein Variants Comment Organism
D361A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D402A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D402E lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D402N lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D404A
-
Abies grandis
D404E lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D404N lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D405A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D405E lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
D405N lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
E499A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
R365A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
R411A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
R454A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
W358A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis
Y520A lower turnover with geranylgeranyl diphosphate than wild-type Abies grandis

Inhibitors

Inhibitors Comment Organism Structure
14,15-dihydro-15-aza-geranylgeranyl diphosphate
-
Abies grandis
geranylgeranyl diphosphate
-
Abies grandis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
geranylgeranyl diphosphate W358A Abies grandis
0.0006
-
geranylgeranyl diphosphate D405N Abies grandis
0.0008
-
geranylgeranyl diphosphate D361A Abies grandis
0.001
-
geranylgeranyl diphosphate D402A, D402E, D405E, R454A Abies grandis
0.002
-
geranylgeranyl diphosphate E499A, R411A, R365A Abies grandis
0.003
-
geranylgeranyl diphosphate wild-type, D405A, Y520A Abies grandis
0.007
-
geranylgeranyl diphosphate D404A Abies grandis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM selectively enabled the conversion of copalyl diphosphate Abies grandis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Abies grandis
-
(+)-copalyl diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Abies grandis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
Abies grandis (+)-copalyl diphosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
geranylgeranyl diphosphate D405N, Y520A Abies grandis
0.2
-
geranylgeranyl diphosphate D402N, R365A, R454A Abies grandis
0.5
-
geranylgeranyl diphosphate R411A Abies grandis
2.2
-
geranylgeranyl diphosphate
-
Abies grandis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
with geranylgeranyl diphosphate as substrate Abies grandis