General Stability | Organism |
---|---|
retains activity after prolonged dialysis | Nocardia sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Nocardia sp. | |
beta-Pinene oxide | - |
Nocardia sp. | |
Carvone | - |
Nocardia sp. | |
limonene oxide | - |
Nocardia sp. | |
p-hydroxymercuribenzoate | - |
Nocardia sp. | |
Pinan-3-ol | - |
Nocardia sp. | |
Pinan-3-one | - |
Nocardia sp. | |
Pinane derivatives with substituent groups at carbon 3 | - |
Nocardia sp. | |
sodium arsenite | weak | Nocardia sp. | |
Sulfhydryl reactive compounds | - |
Nocardia sp. | |
Terpene epoxides | - |
Nocardia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
alpha-Pinene oxide | pH 9.0, 30°C | Nocardia sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Nocardia sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
x * 17000 + x * 22000, SDS-PAGE | Nocardia sp. |
22000 | - |
x * 17000 + x * 22000, SDS-PAGE | Nocardia sp. |
50000 | - |
ultracentrifugal analysis | Nocardia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-pinene oxide | Nocardia sp. | alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene | (Z)-2-methyl-5-isopropylhexa-2,5-dienal | - |
? | |
alpha-pinene oxide | Nocardia sp. P18.3 | alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene | (Z)-2-methyl-5-isopropylhexa-2,5-dienal | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia sp. | - |
- |
- |
Nocardia sp. P18.3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
strain P18.3 | Nocardia sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
alpha-pinene oxide = (Z)-2-methyl-5-isopropylhexa-2,5-dienal | mechanism | Nocardia sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:alpha-pinene-grown cell | - |
Nocardia sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
312 | - |
- |
Nocardia sp. |
Storage Stability | Organism |
---|---|
4°C, 10 days, 7% loss of activity | Nocardia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-pinene oxide | both rings of pinene are cleaved | Nocardia sp. | (Z)-2-methyl-5-isopropylhexa-2,5-dienal | - |
? | |
alpha-pinene oxide | alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene | Nocardia sp. | (Z)-2-methyl-5-isopropylhexa-2,5-dienal | - |
? | |
alpha-pinene oxide | both rings of pinene are cleaved | Nocardia sp. P18.3 | (Z)-2-methyl-5-isopropylhexa-2,5-dienal | - |
? | |
alpha-pinene oxide | alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene | Nocardia sp. P18.3 | (Z)-2-methyl-5-isopropylhexa-2,5-dienal | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 17000 + x * 22000, SDS-PAGE | Nocardia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Nocardia sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
250 | - |
alpha-Pinene oxide | pH 9.0, 30°C | Nocardia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | no significant variation in activity over this range | Nocardia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is devoid of prosthetic groups and has no cofactor requirement | Nocardia sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Nocardia sp. | isoelectric focusing | - |
4 |