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Literature summary for 5.5.1.10 extracted from

  • Griffiths, E.T.; Harries, P.C.; Jeffcoat, R.; Trudgill, P.W.
    Purification and properties of a alpha-pinene oxide lyase from Nocardia sp. strain P18.3 (1987), J. Bacteriol., 169, 4980-4983.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.5.1.10

General Stability

General Stability Organism
retains activity after prolonged dialysis Nocardia sp.

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Nocardia sp.
beta-Pinene oxide
-
 Nocardia sp.
Carvone
-
 Nocardia sp.
limonene oxide
-
 Nocardia sp.
p-hydroxymercuribenzoate
-
 Nocardia sp.
Pinan-3-ol
-
 Nocardia sp.
Pinan-3-one
-
 Nocardia sp.
Pinane derivatives with substituent groups at carbon 3
-
 Nocardia sp.
sodium arsenite weak Nocardia sp.
Sulfhydryl reactive compounds
-
 Nocardia sp.
Terpene epoxides
-
 Nocardia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 alpha-Pinene oxide pH 9.0, 30°C Nocardia sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Nocardia sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 x * 17000 + x * 22000, SDS-PAGE Nocardia sp.
22000
-
 x * 17000 + x * 22000, SDS-PAGE Nocardia sp.
50000
-
 ultracentrifugal analysis Nocardia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-pinene oxide Nocardia sp. alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?
alpha-pinene oxide Nocardia sp. P18.3 alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?

Organism

Organism UniProt Comment Textmining
Nocardia sp.
-
-
-
Nocardia sp. P18.3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strain P18.3 Nocardia sp.

Reaction

Reaction Comment Organism Reaction ID
alpha-pinene oxide = (Z)-2-methyl-5-isopropylhexa-2,5-dienal mechanism Nocardia sp.
a

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:alpha-pinene-grown cell
-
 Nocardia sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
312
-
-
 Nocardia sp.

Storage Stability

Storage Stability Organism
4°C, 10 days, 7% loss of activity Nocardia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-pinene oxide both rings of pinene are cleaved Nocardia sp. (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?
alpha-pinene oxide alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene Nocardia sp. (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?
alpha-pinene oxide both rings of pinene are cleaved Nocardia sp. P18.3 (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?
alpha-pinene oxide alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene Nocardia sp. P18.3 (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?

Subunits

Subunits Comment Organism
? x * 17000 + x * 22000, SDS-PAGE Nocardia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Nocardia sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
250
-
 alpha-Pinene oxide pH 9.0, 30°C Nocardia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 10 no significant variation in activity over this range Nocardia sp.

Cofactor

Cofactor Comment Organism Structure
additional information enzyme is devoid of prosthetic groups and has no cofactor requirement Nocardia sp.

pI Value

Organism Comment pI Value Maximum pI Value
Nocardia sp. isoelectric focusing
-
 4