Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
K169A | 70000fold reduction in activity with cis,cis-muconate, 110000fold reduction in activity with 3-fluoro-cis,cis-muconate, 310fold reduction in activity with 3-chloro-cis,cis-muconate, 2.6fold reduction in activity with 2,4-dichloro-cis,cis-muconate. Wild-type enzyme forms protoanemonin and 2-chloroprotoanemonin from 3-chloro-cis,cis-muconate, mutant enzyme K169A forms cis-dienelactone and 2-chloro-cis-dienelactone | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichloro-cis,cis-muconate | - |
Pseudomonas putida | ? | - |
? | |
3-chloro-cis,cis-muconate | - |
Pseudomonas putida | additional information | wild-type enzyme forms protoanemonin and 2-chloroprotoanemonin, mutant enzyme K169A forms cis-dienelactone and 2-chloro-cis-dienelactone | ? | |
3-Fluoro-cis,cis-muconate | - |
Pseudomonas putida | ? | - |
? | |
cis,cis-muconate | - |
Pseudomonas putida | muconolactone | - |
? |