Literature summary for 5.5.1.1 extracted from

Solyanikova, I.P.; Maltseva, O.V.; Vollmer, M.D.; Golovleva, L.A.; Schl�mann, M.
Characterization of muconate and chloromuconate cycloisomerase from Rhodococcus erythropolis 1CP: indications for functionally convergent evolution among bacterial cycloisomerases (1995), J. Bacteriol., 177, 2821-2826.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0487	-	3-methyl-cis,cis-muconate	-	Rhodococcus erythropolis
0.0559	-	2-chloro-cis,cis-muconate	-	Rhodococcus erythropolis
0.0667	-	2-methyl-cis,cis-muconate	-	Rhodococcus erythropolis
0.0813	-	cis,cis-muconate	-	Rhodococcus erythropolis
1.24	-	3-chloro-cis,cis-muconate	-	Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
230000	250000	nondenaturing electrophoresis, gel filtration	Rhodococcus erythropolis

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus erythropolis	-	1CP	-
Rhodococcus erythropolis 1CP	-	1CP	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rhodococcus erythropolis

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture condition:4-chlorophenol-grown cell	-	Rhodococcus erythropolis	-
culture condition:benzoate-grown cell	-	Rhodococcus erythropolis	-

Storage Stability

Storage Stability	Organism
-20°C, enzyme concentration 0.0035 mg/ml, 50 mM MOPS buffer, pH 6.5, 26% loss of activity after 2 months	Rhodococcus erythropolis
20°C, enzyme concentration 0.0035 mg/ml, 50 mM MOPS buffer, pH 6.5, 69% loss of activity after 2 months	Rhodococcus erythropolis
4°C, enzyme concentration 0.0035 mg/ml, 50 mM MOPS buffer, pH 6.5, no loss of activity after 2 months	Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-Chloro-cis,cis-muconate	-	Rhodococcus erythropolis	?	-	?
2-Chloro-cis,cis-muconate	-	Rhodococcus erythropolis 1CP	?	-	?
2-Methyl-cis,cis-muconate	-	Rhodococcus erythropolis	?	-	?
2-Methyl-cis,cis-muconate	-	Rhodococcus erythropolis 1CP	?	-	?
3-Chloro-cis,cis-muconate	-	Rhodococcus erythropolis	5-Chloromuconolactone	dehalogenation is not catalyzed	?
3-Chloro-cis,cis-muconate	-	Rhodococcus erythropolis 1CP	5-Chloromuconolactone	dehalogenation is not catalyzed	?
3-Methyl-cis,cis-muconate	-	Rhodococcus erythropolis	beta-Methylmuconolactone	-	?
3-Methyl-cis,cis-muconate	-	Rhodococcus erythropolis 1CP	beta-Methylmuconolactone	-	?
cis,cis-Muconate	-	Rhodococcus erythropolis	(+)-Muconolactone	-	?
cis,cis-Muconate	-	Rhodococcus erythropolis 1CP	(+)-Muconolactone	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000-42000, SDS-PAGE	Rhodococcus erythropolis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	30 min, 17% loss of activity	Rhodococcus erythropolis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2	-	3-methyl-cis,cis-muconate	-	Rhodococcus erythropolis
3.2	-	3-methyl-cis,cis-muconate	-	Rhodococcus erythropolis
3.6	-	2-chloro-cis,cis-muconate	-	Rhodococcus erythropolis
8.8	-	3-chloro-cis,cis-muconate	-	Rhodococcus erythropolis
24.7	-	cis,cis-muconate	-	Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Rhodococcus erythropolis

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Release 2023.1 (January 2023)