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Literature summary for 5.5.1.1 extracted from

  • Murakami, S.; Takemoto, J.; Takenaka, S.; Shinke, R.; Aoki, K.
    Purification and characterization of muconate cycloisomerase from aniline-assimilating Rhodococcus erythropolis (1998), J. Ferment. Bioeng., 85, 521-524.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CuSO4
-
Rhodococcus erythropolis
DTNB
-
Rhodococcus erythropolis
EDTA
-
Rhodococcus erythropolis
HgCl2
-
Rhodococcus erythropolis
nitriloacetic acid
-
Rhodococcus erythropolis
PCMB
-
Rhodococcus erythropolis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ is the best metal ion activator Rhodococcus erythropolis
Mn2+ activates to a lower extent than Co2+ Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE Rhodococcus erythropolis
400000
-
gel filtration Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis,cis-Muconate Rhodococcus erythropolis catalyzes the second step of the beta-ketoadipate pathway ?
-
?
cis,cis-Muconate Rhodococcus erythropolis AN-13 catalyzes the second step of the beta-ketoadipate pathway ?
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
AN-13
-
Rhodococcus erythropolis AN-13
-
AN-13
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodococcus erythropolis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rhodococcus erythropolis

Storage Stability

Storage Stability Organism
4°C, pH 7.0, enzyme retains more than 70% of the maximum activity after 24 h Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Methyl-cis,cis-muconate at 6.1% of the activity relative to cis,cis-muconate Rhodococcus erythropolis ?
-
?
2-Methyl-cis,cis-muconate at 6.1% of the activity relative to cis,cis-muconate Rhodococcus erythropolis AN-13 ?
-
?
3-Methyl-cis,cis-muconate at 5.6% of the activity relative to cis,cis-muconate Rhodococcus erythropolis beta-Methylmuconolactone
-
?
3-Methyl-cis,cis-muconate at 5.6% of the activity relative to cis,cis-muconate Rhodococcus erythropolis AN-13 beta-Methylmuconolactone
-
?
cis,cis-Muconate
-
Rhodococcus erythropolis (+)-Muconolactone
-
?
cis,cis-Muconate
-
Rhodococcus erythropolis AN-13 (+)-Muconolactone
-
?
cis,cis-Muconate catalyzes the second step of the beta-ketoadipate pathway Rhodococcus erythropolis ?
-
?
cis,cis-Muconate catalyzes the second step of the beta-ketoadipate pathway Rhodococcus erythropolis AN-13 ?
-
?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Rhodococcus erythropolis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
10 min, less than 20% loss of activity Rhodococcus erythropolis
65
-
10 min, enzyme loses most of its activity Rhodococcus erythropolis