BRENDA - Enzyme Database
show all sequences of 5.4.99.B40

Astertarone A synthase from chinese cabbage does not produce the C4-epimer mechanistic insights

Jin, J.; Moore, M.K.; Wilson, W.K.; Matsuda, S.P.T.; Org. Lett. 20, 1802-1805 (2018)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene bra032185, DNA and amino acid sequence analysis, sequence comparisons, and phylogenetic analysis
Brassica rapa subsp. pekinensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3S)-2,3-epoxy-2,3-dihydrosqualene
Brassica rapa subsp. pekinensis
-
astertarone A
-
-
?
additional information
Brassica rapa subsp. pekinensis
the Brassica rapa oxidosqualene cyclase Bra032185 produces 60% astertarone A and 20 minor triterpenes (0.1-11%), i.e. butyrospermol (11.4%), euphol (8.6%), dammar-21-ene-3beta,18(S)-diol (6.7%), boeticol (4.2%), dammar-21-ene-3beta,18(R)-diol (1.8%), isoeuphol (1.4%), dammar-18(28)-21-dien-3-beta-ol (1.2%), camelliol C (1.0%), and below 1% of isohelianol, 9alphaH-polypoda-7,13E,17E,21-tetraenol, isotirucallol, (20R)--dammara-12,21-dien-3beta-ol, 9alphaH-polypoda-8(26),13E,17E,21-tetraenol, 14-epiarabindiol, (18S)-dammara-12,21-dien-3beta-ol, dammara-18(19)Z-21-dien-3beta-ol, and dammara-18(19)E,21-dien-3-beta-ol
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Brassica rapa subsp. pekinensis
M4ETQ3
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
(3S)-2,3-epoxy-2,3-dihydrosqualene = astertarone A
reaction mechanism via intermediate forms dammarenyl cation, C-4 cation, and enol form, overview
Brassica rapa subsp. pekinensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
748819
Brassica rapa subsp. pekinensis
astertarone A
-
-
-
?
additional information
the Brassica rapa oxidosqualene cyclase Bra032185 produces 60% astertarone A and 20 minor triterpenes (0.1-11%), i.e. butyrospermol (11.4%), euphol (8.6%), dammar-21-ene-3beta,18(S)-diol (6.7%), boeticol (4.2%), dammar-21-ene-3beta,18(R)-diol (1.8%), isoeuphol (1.4%), dammar-18(28)-21-dien-3-beta-ol (1.2%), camelliol C (1.0%), and below 1% of isohelianol, 9alphaH-polypoda-7,13E,17E,21-tetraenol, isotirucallol, (20R)--dammara-12,21-dien-3beta-ol, 9alphaH-polypoda-8(26),13E,17E,21-tetraenol, 14-epiarabindiol, (18S)-dammara-12,21-dien-3beta-ol, dammara-18(19)Z-21-dien-3beta-ol, and dammara-18(19)E,21-dien-3-beta-ol
748819
Brassica rapa subsp. pekinensis
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
Bra032185
-
Brassica rapa subsp. pekinensis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene bra032185, DNA and amino acid sequence analysis, sequence comparisons, and phylogenetic analysis
Brassica rapa subsp. pekinensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3S)-2,3-epoxy-2,3-dihydrosqualene
Brassica rapa subsp. pekinensis
-
astertarone A
-
-
?
additional information
Brassica rapa subsp. pekinensis
the Brassica rapa oxidosqualene cyclase Bra032185 produces 60% astertarone A and 20 minor triterpenes (0.1-11%), i.e. butyrospermol (11.4%), euphol (8.6%), dammar-21-ene-3beta,18(S)-diol (6.7%), boeticol (4.2%), dammar-21-ene-3beta,18(R)-diol (1.8%), isoeuphol (1.4%), dammar-18(28)-21-dien-3-beta-ol (1.2%), camelliol C (1.0%), and below 1% of isohelianol, 9alphaH-polypoda-7,13E,17E,21-tetraenol, isotirucallol, (20R)--dammara-12,21-dien-3beta-ol, 9alphaH-polypoda-8(26),13E,17E,21-tetraenol, 14-epiarabindiol, (18S)-dammara-12,21-dien-3beta-ol, dammara-18(19)Z-21-dien-3beta-ol, and dammara-18(19)E,21-dien-3-beta-ol
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
748819
Brassica rapa subsp. pekinensis
astertarone A
-
-
-
?
additional information
the Brassica rapa oxidosqualene cyclase Bra032185 produces 60% astertarone A and 20 minor triterpenes (0.1-11%), i.e. butyrospermol (11.4%), euphol (8.6%), dammar-21-ene-3beta,18(S)-diol (6.7%), boeticol (4.2%), dammar-21-ene-3beta,18(R)-diol (1.8%), isoeuphol (1.4%), dammar-18(28)-21-dien-3-beta-ol (1.2%), camelliol C (1.0%), and below 1% of isohelianol, 9alphaH-polypoda-7,13E,17E,21-tetraenol, isotirucallol, (20R)--dammara-12,21-dien-3beta-ol, 9alphaH-polypoda-8(26),13E,17E,21-tetraenol, 14-epiarabindiol, (18S)-dammara-12,21-dien-3beta-ol, dammara-18(19)Z-21-dien-3beta-ol, and dammara-18(19)E,21-dien-3-beta-ol
748819
Brassica rapa subsp. pekinensis
?
-
-
-
-
General Information
General Information
Commentary
Organism
evolution
the enzyme is a terpene cyclase/mutase family member, phylogenetic tree
Brassica rapa subsp. pekinensis
additional information
comparison of active-site residues
Brassica rapa subsp. pekinensis
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme is a terpene cyclase/mutase family member, phylogenetic tree
Brassica rapa subsp. pekinensis
additional information
comparison of active-site residues
Brassica rapa subsp. pekinensis
Other publictions for EC 5.4.99.B40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748819
Jin
Astertarone A synthase from c ...
Brassica rapa subsp. pekinensis
Org. Lett.
20
1802-1805
2018
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