Any feedback?
Literature summary for 5.4.99.9 extracted from
- Structural basis of substrate binding to UDP-galactopyranose mutase: crystal structures in the reduced and oxidized state complexed with UDP-galactopyranose and UDP (2009), J. Mol. Biol., 394, 864-877.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Tuner cells | Deinococcus radiodurans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with UDP-galactopyranose and UDP and uridine diphosphate, microbatch under oil method, using 0.1 M HEPES, pH 6.5, 0.2 M lithium chloride and 28% (w/v) PEG 6000 | Deinococcus radiodurans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UDP-D-glucose | poor inhibitor of UGM | Deinococcus radiodurans |  |
| uridine diphosphate | - |
Deinococcus radiodurans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
UDP-galactopyranose | wild type enzyme, in 50 mM sodium phosphate buffer pH 7.0 | Deinococcus radiodurans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus radiodurans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HQ20 column chromatography and HP-20 column chromatography | Deinococcus radiodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactopyranose | - |
Deinococcus radiodurans | UDP-galactofuranose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UGM | - |
Deinococcus radiodurans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 66 | - |
UDP-galactopyranose | wild type enzyme, in 50 mM sodium phosphate buffer pH 7.0 | Deinococcus radiodurans | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains FAD | Deinococcus radiodurans | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1180 | - |
UDP-galactopyranose | wild type enzyme, in 50 mM sodium phosphate buffer pH 7.0 | Deinococcus radiodurans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
