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Literature summary for 5.4.99.9 extracted from

  • Zhang, Q.; Liu, H.w.
    Studies of UDP-galactopyranose mutase from Escherichia coli: An unusual role of reduced FAD in its catalysis (2000), J. Am. Chem. Soc., 122, 9065-9070.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
5-Deazariboflavin specific activity 29fold higher than of control without illumination Escherichia coli
Sodium dithionite 20 mM Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with a His-tag Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
UDP-D-galactofuranose 37°C, pH 7.5, in presence of 20 mM sodium dithionite Escherichia coli
0.194
-
UDP-D-galactofuranose 37°C, pH 7.5 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
alpha2, 2 * 44000, calculated from translated peptide sequence Escherichia coli
72000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-galactopyranose
-
Escherichia coli UDP-D-galactofuranose
-
r

Subunits

Subunits Comment Organism
homodimer alpha2, 2 * 44000, calculated from translated peptide sequence Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
UDP-D-galactofuranose 37°C, pH 7.5 Escherichia coli
27
-
UDP-D-galactofuranose 37°C, pH 7.5, in presence of 20 mM sodium dithionite Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD 0.52 mol bound FAD per monomer Escherichia coli