Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5-Deazariboflavin | specific activity 29fold higher than of control without illumination | Escherichia coli | |
Sodium dithionite | 20 mM | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli with a His-tag | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
UDP-D-galactofuranose | 37°C, pH 7.5, in presence of 20 mM sodium dithionite | Escherichia coli | |
0.194 | - |
UDP-D-galactofuranose | 37°C, pH 7.5 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
alpha2, 2 * 44000, calculated from translated peptide sequence | Escherichia coli |
72000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-galactopyranose | - |
Escherichia coli | UDP-D-galactofuranose | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | alpha2, 2 * 44000, calculated from translated peptide sequence | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
UDP-D-galactofuranose | 37°C, pH 7.5 | Escherichia coli | |
27 | - |
UDP-D-galactofuranose | 37°C, pH 7.5, in presence of 20 mM sodium dithionite | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 0.52 mol bound FAD per monomer | Escherichia coli |