Cloned (Comment) | Organism |
---|---|
cloning of full-length gene WsCAS by homology-based PCR method, DNA and amino acid sequence determination and analysis, sequence comparisons, transformation of overexpressing and RNAi constructs into Withania somnifera plants via Agrobacterium strain GV3101 transformation method, quantitative RT-PCR enzyme expression analysis | Withania somnifera |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of three RNAi gene-silencing constructs in backbone of RNAi vector pGSA and a full-length overexpression construct. The expression of WsCAS gene is considerably downregulated in stable transgenic silenced Withania somnifera lines compared to non-transformed control. Transgenic plants overexpressing CAS gene display enhanced level of CAS transcript and withanolide content compared to non-transformed controls | Withania somnifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene | Withania somnifera | - |
cycloartenol | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Withania somnifera | D6R1X1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Withania somnifera | - |
seedling | - |
Withania somnifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Withania somnifera | cycloartenol | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 83000, about, recombinant enzyme, SDS-PAGE | Withania somnifera |
Synonyms | Comment | Organism |
---|---|---|
(S)-2,3-epoxysqualene mutase | - |
Withania somnifera |
CAS | - |
Withania somnifera |
WsCAS | - |
Withania somnifera |
Organism | Comment | Expression |
---|---|---|
Withania somnifera | wounding, as well as cold or heat treatments downregulate the enzyme expression and winthanolides production in seedlings. Cold, heat and wounding reduce the WsCAS expression levels up to 0.25, 0.51, and 0.59fold respectively. The overall content of withanolide is reduced. Direct correlation between the WsCAS expression and accumulation of withanolide in treated seedlings | down |
Withania somnifera | salicylic acid at 2 mM and 5 mM and methyl jasmonate at 0.2-0.5 mM enhance the WsCAS transcript expression levels 1-2fold. The overall content of withanolide is increased by 1-2fold at 2mM salicylate and by methyl jasmonate at 0.2-0.5 mM. Direct correlation between the WsCAS expression and accumulation of withanolide in treated seedlings under elicitor treatment | up |
General Information | Comment | Organism |
---|---|---|
evolution | cycloartenol synthase belongs to the 2-3 oxidosqualene cyclases (OCSs) gene family | Withania somnifera |
metabolism | the cycloartenol synthase is one of the key enzymes involved in the biosynthesis of withanolides in Withania sominifera. Withanolides are basically of the terpenoid origin and are one of the the largest group of natural products with diverse molecular structures. Cycloartenol which acts as the key precursor for the biosynthesis of phytosterols as well as withanolides through a series of desaturation, hydroxylation, epoxidations, cyclization, chain elongation, and glycosylation steps. Withanolide biosynthetic pathway, overview | Withania somnifera |