Cloned (Comment) | Organism |
---|---|
isozyme CAS1, RNA-sequencing analysis from seedling genome, cloning from cDNA library, DNA and amino acid sequence determination and analysis, isozyme sequence comparison, phylogenetic tree of OSCs, quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae, GC-MS analysis of cycloartenol in yeast | Polygala tenuifolia |
isozyme CAS2, RNA-sequencing analysis from seedling genome, cloning from cDNA library, DNA and amino acid sequence determination and analysis, isozyme sequence comparison, phylogenetic tree of OSCs, quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae, GC-MS analysis of cycloartenol in yeast | Polygala tenuifolia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene | Polygala tenuifolia | - |
cycloartenol | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Polygala tenuifolia | A9QW75 | - |
- |
Polygala tenuifolia | A9QW77 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Polygala tenuifolia | - |
additional information | the expression of PtCAS1 is highest in flowers | Polygala tenuifolia | - |
additional information | the expression of PtCAS2 is highest in roots | Polygala tenuifolia | - |
root | - |
Polygala tenuifolia | - |
seedling | - |
Polygala tenuifolia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Polygala tenuifolia | cycloartenol | - |
r |
Synonyms | Comment | Organism |
---|---|---|
OSC | - |
Polygala tenuifolia |
PtCAS1 | - |
Polygala tenuifolia |
PtCAS2 | - |
Polygala tenuifolia |
Organism | Comment | Expression |
---|---|---|
Polygala tenuifolia | the isozyme PtCAS1 is induced 1.5fold by methyl jasmonate. The transcript patterns of the two PtCAS isozyme seedling genes after methyl jasmonate treatment are similar | up |
Polygala tenuifolia | the isozyme PtCAS2 is induced 2.0fold by methyl jasmonate. The transcript patterns of the two PtCAS isozyme seedling genes after methyl jasmonate treatment are similar | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the oxidosqualene cyclases (OSCs), enzymes that play a key role in control of the biosynthesis of phytosterols and triterpene saponins | Polygala tenuifolia |
physiological function | CAS s play a vital role in sterol biosynthesis, which is essential for plant cell viability | Polygala tenuifolia |