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Literature summary for 5.4.99.7 extracted from

  • Wu, T.K.; Chang, C.H.; Wen, H.Y.; Liu, Y.T.; Li, W.H.; Wang, T.T.; Shie, W.S.
    Alteration of the substrates prefolded conformation and cyclization stereochemistry of oxidosqualene-lanosterol cyclase of Saccharomyces cerevisiae by substitution at phenylalanine 699 (2010), Org. Lett., 12, 500-503.
    View publication on PubMed
Search for more literature for 5.4.99.7

Protein Variants

Protein Variants Comment Organism
F699H the mutant produces lanosterol (13%), protosta-13(17)-dien-3beta-ol (70%), and (17Z)-protosta-17(20),24-dien-3beta-ol (17%) Saccharomyces cerevisiae
F699I the mutant produces lanosterol (100%) as the wild type enzyme Saccharomyces cerevisiae
F699L the mutant produces lanosterol (100%) as the wild type enzyme Saccharomyces cerevisiae
F699M the mutant produces lanosterol (13%), (17Z)-protosta-17(20),24-dien-3beta-ol (46%), protosta-13(17)-dien-3beta-ol (70%), malabarica-14E,17E,21-trien-3beta-ol (7%), 17alpha-protosta-20,24-dien-3beta-ol (1%), (17Z)-protosta-17(20),24-dien-3beta-ol (10%), (13alphaH)-isomalabarica-14E,17E,21-dien-3 beta-ol (17%), and (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol (18%) Saccharomyces cerevisiae
F699N nonviable mutant, does not produce lanosterol, but protosta-13(17)-dien-3beta-ol (55%), malabarica-14E,17E,21-trien-3beta-ol (5%), 17alpha-protosta-20,24-dien-3beta-ol (24%), and (17Z)-protosta-17(20),24-dien-3beta-ol (16%) Saccharomyces cerevisiae
F699P the mutant produces lanosterol (100%) as the wild type enzyme Saccharomyces cerevisiae
F699T the mutant produces lanosterol (less than 0.2%) and protosta-13(17)-dien-3beta-ol (above 99.8%) Saccharomyces cerevisiae
F699X TKW14[pERG7F699X] site-saturated mutants allow for ergosterol-independent growth, with the exception of Leu, Ile, His, Met, Pro, and Thr substitutions Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae TKW14
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-2,3-oxidosqualene
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 Saccharomyces cerevisiae lanosterol
-
 ?
(3S)-2,3-oxidosqualene
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 Saccharomyces cerevisiae TKW14 lanosterol
-
 ?

Synonyms

Synonyms Comment Organism
ERG7
-
 Saccharomyces cerevisiae
OSC
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 Saccharomyces cerevisiae
Oxidosqualene-lanosterol cyclase
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 Saccharomyces cerevisiae