Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C457D | less active than the wild-type | Saccharomyces cerevisiae |
C457D/A525C | similar activity as the wild-type | Saccharomyces cerevisiae |
C457D/E526A | decrease in enzyme functionality | Saccharomyces cerevisiae |
C457D/E526C | very sensitive to the thiol-reacting agent dodecylmaleimide, specific activity and thermal stability are severely reduced | Saccharomyces cerevisiae |
C457D/E526D | no effect on catalytic avtivity | Saccharomyces cerevisiae |
C457D/E526Q | no effect on catalytic avtivity | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
mutants C457D/E526C and C457D/E526A are not stable at 35°C | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dodecyl-maleimide | mutant C457D shows 41.6% inhibition at 1 mM, 10.0% at 0.2 mM, and 7.2% at 0.025 mM, mutant C457D/E526A shows 9.0% inhibition at 0.2 mM and 0.0% at 0.025 mM, mutant C457D/A525C shows 86.0% inhibition at 1 mM, 30.5% at 0.2 mM, and 1.0% at 0.025 mM, mutant C457D/E526C shows 89.6% inhibition at 0.2 mM, 74.7% at 0.025 mM, and 45.1% at 0.01 mM | Saccharomyces cerevisiae | |
N,N-Dimethylformamide | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-oxidosqualene | Saccharomyces cerevisiae | - |
lanosterol | - |
? | |
(3S)-2,3-oxidosqualene | Saccharomyces cerevisiae SMY8 | - |
lanosterol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae SMY8 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000015 | - |
mutant C457/E526A at 25°C | Saccharomyces cerevisiae |
0.0000015 | - |
mutant C457/E526C at 25°C | Saccharomyces cerevisiae |
0.0000025 | - |
mutant C457/E526C at 35°C | Saccharomyces cerevisiae |
0.0000048 | - |
mutant C457/E526A at 35°C | Saccharomyces cerevisiae |
0.0000063 | - |
mutant C457 at 25°C | Saccharomyces cerevisiae |
0.0000087 | - |
mutant C457/E526D at 25°C | Saccharomyces cerevisiae |
0.0000097 | - |
mutant C457/E526Q at 25°C | Saccharomyces cerevisiae |
0.0000113 | - |
mutant C457/E525C at 25°C | Saccharomyces cerevisiae |
0.0000128 | - |
mutant C457/E526D at 35°C | Saccharomyces cerevisiae |
0.0000172 | - |
wild-type, at 25°C | Saccharomyces cerevisiae |
0.0000192 | - |
mutant C457/E526Q at 35°C | Saccharomyces cerevisiae |
0.000021 | - |
mutant C457/E525C at 35°C | Saccharomyces cerevisiae |
0.0000213 | - |
mutant C457 at 35°C | Saccharomyces cerevisiae |
0.0000292 | - |
wild-type, at 35°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-oxidosqualene | - |
Saccharomyces cerevisiae | lanosterol | - |
? | |
(3S)-2,3-oxidosqualene | - |
Saccharomyces cerevisiae SMY8 | lanosterol | - |
? | |
squalene | - |
Saccharomyces cerevisiae | ? | - |
? | |
squalene | - |
Saccharomyces cerevisiae SMY8 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lanosterol synthase | - |
Saccharomyces cerevisiae |
OSC | - |
Saccharomyces cerevisiae |
Oxidosqualene cyclase | - |
Saccharomyces cerevisiae |
SceOSC | - |
Saccharomyces cerevisiae |