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Literature summary for 5.4.99.7 extracted from

  • Wu, T.K.; Huang, C.Y.; Ko, C.Y.; Chang, C.H.; Chen, Y.J.; Liao, H.K.
    Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver (2004), Arch. Biochem. Biophys., 421, 42-53.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate maximal inactivation at neutral pH, ´reactivation by hydroxylamine Bos taurus
N,N-dimethyl-N-(4-methylenebenzophenonyl)amine IC50: 0.005 mM Bos taurus
N-(2-methylenebenzophenonyl)pyridinium IC50: 0.059 mM Bos taurus
N-(3-methylenebenzophenonyl)pyridinium bromide IC50: 0.016 mM Bos taurus
N-(4-methylenebenzophenonyl)pyridinium bromide IC50: 0.00079 mM Bos taurus
Ro48-8071 IC50: 22 nM Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
(3S)-2,3-oxidosqualene
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
2 * 70000, SDS-PAGE Bos taurus
140000
-
non-denaturing PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.747
-
-
Bos taurus

Storage Stability

Storage Stability Organism
-20°C or -80°C, phosphate buffer, in presence of Triton and DTT, stable for up to 6 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-2,3-oxidosqualene two essential histidine residues per active site are necessary for complete activity Bos taurus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 70000, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
OSC
-
Bos taurus
Oxidosqualene-lanosterol cyclase
-
Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Bos taurus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 optimal activity between 25°C and 50°C with a maximum at 37°C Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 20% of maximal activity, pH 7.0: about 90% of maximal activity, pH 9.0: about 50% of maximal activity Bos taurus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000022
-
IC50: 22 nM Bos taurus Ro48-8071
0.00079
-
IC50: 0.00079 mM Bos taurus N-(4-methylenebenzophenonyl)pyridinium bromide
0.005
-
IC50: 0.005 mM Bos taurus N,N-dimethyl-N-(4-methylenebenzophenonyl)amine
0.016
-
IC50: 0.016 mM Bos taurus N-(3-methylenebenzophenonyl)pyridinium bromide
0.059
-
IC50: 0.059 mM Bos taurus N-(2-methylenebenzophenonyl)pyridinium