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Literature summary for 5.4.99.65 extracted from

  • Saga, Y.; Araki, T.; Araya, H.; Saito, K.; Yamazaki, M.; Suzuki, H.; Kushiro, T.
    Identification of serratane synthase gene from the fern Lycopodium clavatum (2017), Org. Lett., 19, 496-499 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functional recombinant enzyme expression in Saccharomyces cerevisiae strain GIL77, coexpression with alpha-onocerin synthase leading to the production of tohogenol and serratenediol, NMR metabolite identification, overview Lycopodium clavatum

Protein Variants

Protein Variants Comment Organism
additional information while the heterologous LCC gene expression produces the bicyclic triterpene pre-alpha-onocerin, a combination of LCC and alpha-onocerin synthase LCD produces onoceranes, and that of LCC and tohogenol synthase LCE produces serratanes Lycopodium clavatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3S,22S)-2,3:22,23-diepoxy-2,3,22,23-tetrahydrosqualene Lycopodium clavatum
-
pre-alpha-onocerin
-
?

Organism

Organism UniProt Comment Textmining
Lycopodium clavatum A0A125SXN1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S,22S)-2,3:22,23-diepoxy-2,3,22,23-tetrahydrosqualene
-
Lycopodium clavatum pre-alpha-onocerin
-
?

Synonyms

Synonyms Comment Organism
Lcc
-
Lycopodium clavatum

General Information

General Information Comment Organism
metabolism ferns are known to produce onoceroids including onoceranes and serratanes having unusual structures among triterpenes. Serratanes are directly biosynthesized from pre-alpha-onocerin, synthesized by pre-alpha-onocerin synthase, and not from alpha-onocerin, synthesized by alpha-onocerin synthase, EC 5.4.99.66, from pre-alpha-onocerin Lycopodium clavatum
physiological function ferns are known to produce onoceroids including onoceranes and serratanes having unusual structures among triterpenes. Serratanes are directly biosynthesized from pre-alpha-onocerin, synthesized by pre-alpha-onocerin synthase, and not from alpha-onocerin, synthesized by alpha-onocerin synthase, EC 5.4.99.66, from pre-alpha-onocerin Lycopodium clavatum