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Literature summary for 5.4.99.64 extracted from

  • Kurteva-Yaneva, N.; Zahn, M.; Weichler, M.T.; Starke, R.; Harms, H.; Muller, R.H.; Strater, N.; Rohwerder, T.
    Structural basis of the stereospecificity of bacterial B12-dependent 2-hydroxyisobutyryl-CoA mutase (2015), J. Biol. Chem., 290, 9727-9737.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.64

Cloned(Commentary)

Cloned (Comment) Organism
-
 Aquincola tertiaricarbonis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure in complex with coenzyme B12 and the substrates (S)-3-hydroxybutyryl- and 2-hydroxyisobutyryl-CoA in alternative binding. subunit HcmA residues IleA90 and AspA117. AspA117 determine the orientation of the hydroxyl group of the acyl-CoA esters by H-bond formation, thus determining stereospecificity of catalysis Aquincola tertiaricarbonis
crystal structure of the enzyme in complex with coenzyme B12 and the substrates (S)-3-hydroxybutyryl- and 2-hydroxyisobutyryl-CoA in alternative binding. Crystals are grown in 16% PEG 3350, 0.1 M Tris,pH 7.5, by the hanging-drop vapor diffusion method at 4°C Aquincola tertiaricarbonis

Protein Variants

Protein Variants Comment Organism
D117A mutation in the large subunit HcmA results in significantly increased activity toward (R)-3-hydroxybutyryl-CoA Aquincola tertiaricarbonis
D117A mutation in subunit HcmA, results in significantly increased activity toward (R)-3-hydroxybutyryl-CoA Aquincola tertiaricarbonis
D117V mutation in the large subunit HcmA results in significantly increased activity toward (R)-3-hydroxybutyryl-CoA. The mutant enzyme shows high activity toward pivalyl-CoA Aquincola tertiaricarbonis
D117V mutation in subunit HcmA, results in significantly increased activity toward (R)-3-hydroxybutyryl-CoA and high activity toward pivalyl-CoA Aquincola tertiaricarbonis
I90A strong decrease in activity Aquincola tertiaricarbonis
I90A mutation in the large subunit HcmA results in a significant reduction in conversion rates and diminution of the catalytic efficiencies for the main substrates of the wild-type enzyme, (S)-3-hydroxybutyryl-coA and 2-hydroxyisobutyryl-CoA. Wild-type enzyme as well as mutant enzyme can also isomerize pivalyl- and isovaleryl-CoA, albeit at more than 10times lower rates than the favorite substrate (S)-3-hydroxybutyryl-CoA Aquincola tertiaricarbonis
I90L strong decrease in activity Aquincola tertiaricarbonis
I90L mutation in the large subunit HcmA results in a significant reduction in conversion rates and diminution of the catalytic efficiencies for the main substrates of the wild-type enzyme, (S)-3-hydroxybutyryl-coA and 2-hydroxyisobutyryl-CoA. Mutant enzyme does not show any significant activities with pivalyl-CoA and isovaleryl-CoA Aquincola tertiaricarbonis
I90V strong decrease in activity Aquincola tertiaricarbonis
I90V mutation in the large subunit HcmA, wild-type enzyme as well as mutant enzyme can also isomerize pivalyl- and isovaleryl-CoA, albeit at more than 10times lower rates than the favorite substrate (S)-3-hydroxybutyryl-CoA Aquincola tertiaricarbonis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0075
-
 (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.008
-
 (R)-3-hydroxybutanoyl-CoA mutant D117A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.0146
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.015
-
 (S)-3-hydroxybutanoyl-CoA mutant D117A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.042
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.042
-
 2-hydroxy-2-methylpropanoyl-CoA mutant D117A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.0435
-
 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.061
-
 2-hydroxy-2-methylpropanoyl-CoA mutant D117V, pH 6.6, 30°C Aquincola tertiaricarbonis
0.0614
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.067
-
 (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.067
-
 (R)-3-hydroxybutanoyl-CoA mutant D117V, pH 6.6, 30°C Aquincola tertiaricarbonis
0.104
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.104
-
 2-hydroxy-2-methylpropanoyl-CoA wild-type, pH 6.6, 30°C Aquincola tertiaricarbonis
0.117
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.117
-
 (S)-3-hydroxybutanoyl-CoA mutant D117V, pH 6.6, 30°C Aquincola tertiaricarbonis
0.128
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.128
-
 (S)-3-hydroxybutanoyl-CoA wild-type, pH 6.6, 30°C Aquincola tertiaricarbonis
0.147
-
 isovaleryl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.235
-
 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.288
-
 isovaleryl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.298
-
 (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.298
-
 (R)-3-hydroxybutanoyl-CoA mutant I90A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.304
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.304
-
 2-hydroxy-2-methylpropanoyl-CoA mutant I90A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.329
-
 isovaleryl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.374
-
 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.433
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme, I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.433
-
 (S)-3-hydroxybutanoyl-CoA mutant I90A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.517
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90L (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.517
-
 2-hydroxy-2-methylpropanoyl-CoA mutant I90L, pH 6.6, 30°C Aquincola tertiaricarbonis
0.663
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90L (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.663
-
 (S)-3-hydroxybutanoyl-CoA mutant I90L, pH 6.6, 30°C Aquincola tertiaricarbonis
0.977
-
 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) Aquincola tertiaricarbonis
1.66
-
 (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
1.66
-
 (R)-3-hydroxybutanoyl-CoA wild-type, pH 6.6, 30°C Aquincola tertiaricarbonis
1.76
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) Aquincola tertiaricarbonis
1.76
-
 (S)-3-hydroxybutanoyl-CoA mutant I90V, pH 6.6, 30°C Aquincola tertiaricarbonis
1.84
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) Aquincola tertiaricarbonis
1.84
-
 2-hydroxy-2-methylpropanoyl-CoA mutant I90V, pH 6.6, 30°C Aquincola tertiaricarbonis

Organism

Organism UniProt Comment Textmining
Aquincola tertiaricarbonis I3VE77 and I3VE74 I3VE77: large subunit hcmA, I3VE74: small subunit hcmB
-
Aquincola tertiaricarbonis I3VE77 and I3VE74 I3VE77, i.e. large subunit HcmA, I3VE74, i.e. small subunit HcmB, respectively
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aquincola tertiaricarbonis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutanoyl-CoA poor substrate Aquincola tertiaricarbonis 2-hydroxy-2-methylpropanoyl-CoA
-
 r
(R)-3-hydroxybutanoyl-CoA very low activity with wild-type enzyme. Mutations D117V or D117A in the large subunit HcmA result in significantly increased activity toward (R)-3-hydroxybutyryl-CoA Aquincola tertiaricarbonis 2-hydroxy-2-methylpropanoyl-CoA
-
 r
(S)-3-hydroxybutanoyl-CoA
-
 Aquincola tertiaricarbonis 2-hydroxy-2-methylpropanoyl-CoA
-
 r
(S)-3-hydroxybutanoyl-CoA favorite substrate Aquincola tertiaricarbonis 2-hydroxy-2-methylpropanoyl-CoA
-
 r
2,2-dimethylpropanoyl-CoA wild-type enzyme as well as I90V (HcmA) and I90A (HcmA) mutant enzymes can also isomerize pivaloyl-CoA and isovaleryl-CoA, albeit at more than 10times lower rates than the favorite substrate (S)-3-hydroxybutyryl-CoA Aquincola tertiaricarbonis ?
-
 r
2-hydroxy-2-methylpropanoyl-CoA
-
 Aquincola tertiaricarbonis (S)-3-hydroxybutanoyl-CoA
-
 r
isobutanoyl-CoA
-
 Aquincola tertiaricarbonis butanoyl-CoA
-
 r
isovaleryl-CoA wild-type enzyme as well as I90V (HcmA) and I90A (HcmA) mutant enzymes can also isomerize pivalyl- and isovaleryl-CoA, albeit at more than 10times lower rates than the favorite substrate (S)-3-hydroxybutyryl-CoA Aquincola tertiaricarbonis ?
-
 ?
isovaleryl-CoA at 10 times lower rate than substrate (S)-3-hydroxybutanoyl-CoA Aquincola tertiaricarbonis pentanoyl-CoA
-
 r
methylmalonyl-CoA
-
 Aquincola tertiaricarbonis succinyl-CoA
-
 r
pivaloyl-CoA at 10 times lower rate than substrate (S)-3-hydroxybutanoyl-CoA Aquincola tertiaricarbonis ?
-
 r

Synonyms

Synonyms Comment Organism
2-hydroxyisobutyryl-CoA mutase
-
 Aquincola tertiaricarbonis
HCM
-
 Aquincola tertiaricarbonis
hcmAB
-
 Aquincola tertiaricarbonis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Aquincola tertiaricarbonis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.002
-
 (S)-3-hydroxybutanoyl-CoA mutant I90A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.003
-
 (S)-3-hydroxybutanoyl-CoA mutant I90L, pH 6.6, 30°C Aquincola tertiaricarbonis
0.003
-
 (R)-3-hydroxybutanoyl-CoA wild-type, pH 6.6, 30°C Aquincola tertiaricarbonis
0.0033
-
 (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.0035
-
 (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.0035
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90L (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.004
-
 (R)-3-hydroxybutanoyl-CoA mutant I90A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.0067
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90L (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.007
-
 2-hydroxy-2-methylpropanoyl-CoA mutant I90L, pH 6.6, 30°C Aquincola tertiaricarbonis
0.009
-
 isovaleryl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.0093
-
 isovaleryl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.011
-
 isovaleryl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.013
-
 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.016
-
 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.017
-
 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.02
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.02
-
 2-hydroxy-2-methylpropanoyl-CoA mutant I90V, pH 6.6, 30°C Aquincola tertiaricarbonis
0.0245
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.025
-
 2-hydroxy-2-methylpropanoyl-CoA mutant I90A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.0255
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.026
-
 (S)-3-hydroxybutanoyl-CoA mutant D117A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.027
-
 (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.027
-
 (R)-3-hydroxybutanoyl-CoA mutant D117A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.033
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.033
-
 (S)-3-hydroxybutanoyl-CoA mutant I90V, pH 6.6, 30°C Aquincola tertiaricarbonis
0.044
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.044
-
 2-hydroxy-2-methylpropanoyl-CoA mutant D117A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.045
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.045
-
 2-hydroxy-2-methylpropanoyl-CoA mutant D117V, pH 6.6, 30°C Aquincola tertiaricarbonis
0.05
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.05
-
 2-hydroxy-2-methylpropanoyl-CoA wild-type, pH 6.6, 30°C Aquincola tertiaricarbonis
0.062
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.062
-
 (S)-3-hydroxybutanoyl-CoA mutant D117V, pH 6.6, 30°C Aquincola tertiaricarbonis
0.14
-
 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.17
-
 (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.173
-
 (R)-3-hydroxybutanoyl-CoA mutant D117V, pH 6.6, 30°C Aquincola tertiaricarbonis
0.2
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.2
-
 (S)-3-hydroxybutanoyl-CoA wild-type, pH 6.6, 30°C Aquincola tertiaricarbonis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
 assay at Aquincola tertiaricarbonis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.002
-
 (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.002
-
 (R)-3-hydroxybutanoyl-CoA wild-type, pH 6.6, 30°C Aquincola tertiaricarbonis
0.0047
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme, I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.005
-
 (S)-3-hydroxybutanoyl-CoA mutant I90A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.005
-
 (S)-3-hydroxybutanoyl-CoA mutant I90L, pH 6.6, 30°C Aquincola tertiaricarbonis
0.0052
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90L (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.012
-
 (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.012
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.012
-
 (R)-3-hydroxybutanoyl-CoA mutant I90A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.012
-
 2-hydroxy-2-methylpropanoyl-CoA mutant I90V, pH 6.6, 30°C Aquincola tertiaricarbonis
0.013
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90L (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.013
-
 2-hydroxy-2-methylpropanoyl-CoA mutant I90L, pH 6.6, 30°C Aquincola tertiaricarbonis
0.017
-
 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.018
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.018
-
 (S)-3-hydroxybutanoyl-CoA mutant I90V, pH 6.6, 30°C Aquincola tertiaricarbonis
0.028
-
 isovaleryl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.04
-
 isovaleryl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.043
-
 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.057
-
 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.062
-
 isovaleryl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.082
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.082
-
 2-hydroxy-2-methylpropanoyl-CoA mutant I90A, pH 6.6, 30°C Aquincola tertiaricarbonis
0.48
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
0.48
-
 2-hydroxy-2-methylpropanoyl-CoA wild-type, pH 6.6, 30°C Aquincola tertiaricarbonis
0.525
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.525
-
 (S)-3-hydroxybutanoyl-CoA mutant D117V, pH 6.6, 30°C Aquincola tertiaricarbonis
0.727
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
0.727
-
 2-hydroxy-2-methylpropanoyl-CoA mutant D117V, pH 6.6, 30°C Aquincola tertiaricarbonis
1.05
-
 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) Aquincola tertiaricarbonis
1.053
-
 2-hydroxy-2-methylpropanoyl-CoA mutant D117A, pH 6.6, 30°C Aquincola tertiaricarbonis
1.5
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, wild-type enzyme Aquincola tertiaricarbonis
1.5
-
 (S)-3-hydroxybutanoyl-CoA wild-type, pH 6.6, 30°C Aquincola tertiaricarbonis
1.75
-
 (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) Aquincola tertiaricarbonis
1.75
-
 (S)-3-hydroxybutanoyl-CoA mutant D117A, pH 6.6, 30°C Aquincola tertiaricarbonis
2.583
-
 (R)-3-hydroxybutanoyl-CoA mutant D117V, pH 6.6, 30°C Aquincola tertiaricarbonis
2.6
-
 (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
3.2
-
 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) Aquincola tertiaricarbonis
3.667
-
 (R)-3-hydroxybutanoyl-CoA mutant D117A, pH 6.6, 30°C Aquincola tertiaricarbonis
3.7
-
 (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) Aquincola tertiaricarbonis