Application | Comment | Organism |
---|---|---|
synthesis | synthesis of 2-hydroxyisobutanoate in Cupriavidus necator H16 expressing mutase genes HcmA and HcmB. Due to the enantiospecificity of the mutase, fructose is a weaker substrate for 2-hydroxyisobutanoate synthesis than butanoate. Production rates achieved with the poly-3-hydroxybutanoate-negative strain H16 PHB?4 on butanoate are higher than on fructose. Using the wild-type does not significantly improve the production rates, the latter shows a 34fold and a 5fold lower 2-hydroxyisobutanoate synthesis rate compared to H16 PHB?4 on fructose and butanoate, respectively. Both strains show concomitant excretion of undesired side products, such as pyruvate and 3-hydroxybutanoate | Aquincola tertiaricarbonis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquincola tertiaricarbonis | I3VE77 and I3VE74 | I3VE77 i.e. large subunit HcmA, I3VE74 i.e. small subunit HcmB | - |
Aquincola tertiaricarbonis L108 | I3VE77 and I3VE74 | I3VE77 i.e. large subunit HcmA, I3VE74 i.e. small subunit HcmB | - |
Synonyms | Comment | Organism |
---|---|---|
HcmA | - |
Aquincola tertiaricarbonis |
HcmB | - |
Aquincola tertiaricarbonis |