BRENDA - Enzyme Database
show all sequences of 5.4.99.50

Friedelin in Maytenus ilicifolia is produced by friedelin synthase isoforms

Alves, T.B.; Souza-Moreira, T.M.; Valentini, S.R.; Zanelli, C.F.; Furlan, M.; Molecules 23, 700-711 (2018)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
DNA and amino acid sequence determination and analysis of isozymes, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae strain VZL1303; DNA and amino acid sequence determination and analysis of isozymes, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae strain VZL1303; DNA and amino acid sequence determination and analysis of isozymes, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae strain VZL1303; DNA and amino acid sequence determination and analysis of isozymes, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae strain VZL1303
Monteverdia ilicifolia
Engineering
Protein Variants
Commentary
Organism
A48V
naturally occuring mutation, single nucleotide exchange; naturally occuring mutation, single nucleotide exchange
Monteverdia ilicifolia
E309K
naturally occuring mutation, single nucleotide exchange
Monteverdia ilicifolia
I768K
naturally occuring mutation, single nucleotide exchange
Monteverdia ilicifolia
additional information
presence of single nucleotide polymorphisms with four non-synonymous mutations outside the active site of isozyme MiFRS
Monteverdia ilicifolia
P39S
naturally occuring mutation, single nucleotide exchange
Monteverdia ilicifolia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3S)-2,3-epoxy-2,3-dihydrosqualene
Monteverdia ilicifolia
-
friedelin
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Monteverdia ilicifolia
A0A1L3HE30
-
-
Monteverdia ilicifolia
A0A2R3ZF24
-
-
Monteverdia ilicifolia
A0A2R3ZF26
-
-
Monteverdia ilicifolia
A0A2R3ZF31
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
(3S)-2,3-epoxy-2,3-dihydrosqualene = friedelin
reaction via a chair-chair-chair conformation intermediate; reaction via a chair-chair-chair conformation intermediate; reaction via a chair-chair-chair conformation intermediate; reaction via a chair-chair-chair conformation intermediate
Monteverdia ilicifolia
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Monteverdia ilicifolia
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
the four isozymes of the enzyme show different specific activities; the four isozymes of the enzyme show different specific activities; the four isozymes of the enzyme show different specific activities; the four isozymes of the enzyme show different specific activities
Monteverdia ilicifolia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
748722
Monteverdia ilicifolia
friedelin
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 89160, about, sequence calculation; x * 89160, about, sequence calculation; x * 89170, about, sequence calculation; x * 89170, about, sequence calculation
Monteverdia ilicifolia
Synonyms
Synonyms
Commentary
Organism
FRS
-
Monteverdia ilicifolia
FRS2
-
Monteverdia ilicifolia
FRS3
-
Monteverdia ilicifolia
MiFRS
-
Monteverdia ilicifolia
MiFRS2
-
Monteverdia ilicifolia
MiFRS3
-
Monteverdia ilicifolia
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Monteverdia ilicifolia
sequence calculation
-
6.04
Monteverdia ilicifolia
sequence calculation; sequence calculation
-
6.17
Monteverdia ilicifolia
sequence calculation
-
6.24
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis of isozymes, sequence comparisons and phylogenetic analysis, functional recombinant expression in Saccharomyces cerevisiae strain VZL1303
Monteverdia ilicifolia
Engineering (protein specific)
Protein Variants
Commentary
Organism
A48V
naturally occuring mutation, single nucleotide exchange
Monteverdia ilicifolia
E309K
naturally occuring mutation, single nucleotide exchange
Monteverdia ilicifolia
I768K
naturally occuring mutation, single nucleotide exchange
Monteverdia ilicifolia
additional information
presence of single nucleotide polymorphisms with four non-synonymous mutations outside the active site of isozyme MiFRS
Monteverdia ilicifolia
P39S
naturally occuring mutation, single nucleotide exchange
Monteverdia ilicifolia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3S)-2,3-epoxy-2,3-dihydrosqualene
Monteverdia ilicifolia
-
friedelin
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Monteverdia ilicifolia
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
the four isozymes of the enzyme show different specific activities
Monteverdia ilicifolia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
748722
Monteverdia ilicifolia
friedelin
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 89160, about, sequence calculation
Monteverdia ilicifolia
?
x * 89170, about, sequence calculation
Monteverdia ilicifolia
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Monteverdia ilicifolia
sequence calculation
-
6.04
Monteverdia ilicifolia
sequence calculation
-
6.17
Monteverdia ilicifolia
sequence calculation
-
6.24
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the terpenoid cyclases/protein prenyltransferases superfamily. Characterictic motifs for the OSC family and friedelin synthase are the four structural QW motifs (aromatic rich motifs of seven residues from Gln to Trp), the cavity site, and the Leu482. All of them are important for product specificity; the enzyme belongs to the terpenoid cyclases/protein prenyltransferases superfamily. Characterictic motifs for the OSC family and friedelin synthase are the four structural QW motifs (aromatic rich motifs of seven residues from Gln to Trp), the cavity site, and the Leu482. All of them are important for product specificity; the enzyme belongs to the terpenoid cyclases/protein prenyltransferases superfamily. Characterictic motifs for the OSC family and friedelin synthase are the four structural QW motifs (aromatic rich motifs of seven residues from Gln to Trp), the cavity site, and the Leu482. All of them are important for product specificity; the enzyme belongs to the terpenoid cyclases/protein prenyltransferases superfamily. Characterictic motifs for the OSC family and friedelin synthase are the four structural QW motifs (aromatic rich motifs of seven residues from Gln to Trp), the cavity site, and the Leu482. All of them are important for product specificity
Monteverdia ilicifolia
metabolism
the enzyme is involved in the biosynthesis of steroidal and pentacyclic triterpenes, overview. Friedelin is a precursor for other triterpenes and quinonemethides; the enzyme is involved in the biosynthesis of steroidal and pentacyclic triterpenes, overview. Friedelin is a precursor for other triterpenes and quinonemethides; the enzyme is involved in the biosynthesis of steroidal and pentacyclic triterpenes, overview. Friedelin is a precursor for other triterpenes and quinonemethides; the enzyme is involved in the biosynthesis of steroidal and pentacyclic triterpenes, overview. Friedelin is a precursor for other triterpenes and quinonemethides
Monteverdia ilicifolia
physiological function
the enzyme synthesizes friedelin, a triterpene ketone with anti-inflammatory and gastroprotective activities and it is a precursor of relevant antitumor quinonemethides; the enzyme synthesizes friedelin, a triterpene ketone with anti-inflammatory and gastroprotective activities and it is a precursor of relevant antitumor quinonemethides; the enzyme synthesizes friedelin, a triterpene ketone with anti-inflammatory and gastroprotective activities and it is a precursor of relevant antitumor quinonemethides; the enzyme synthesizes friedelin, a triterpene ketone with anti-inflammatory and gastroprotective activities and it is a precursor of relevant antitumor quinonemethides
Monteverdia ilicifolia
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the terpenoid cyclases/protein prenyltransferases superfamily. Characterictic motifs for the OSC family and friedelin synthase are the four structural QW motifs (aromatic rich motifs of seven residues from Gln to Trp), the cavity site, and the Leu482. All of them are important for product specificity
Monteverdia ilicifolia
metabolism
the enzyme is involved in the biosynthesis of steroidal and pentacyclic triterpenes, overview. Friedelin is a precursor for other triterpenes and quinonemethides
Monteverdia ilicifolia
physiological function
the enzyme synthesizes friedelin, a triterpene ketone with anti-inflammatory and gastroprotective activities and it is a precursor of relevant antitumor quinonemethides
Monteverdia ilicifolia
Other publictions for EC 5.4.99.50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748722
Alves
Friedelin in Maytenus ilicifo ...
Monteverdia ilicifolia
Molecules
23
700-711
2018
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1
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5
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6
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1
1
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4
1
8
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6
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4
4
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4
4
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4
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3
12
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749326
Souza-Moreira
Friedelin synthase from Mayte ...
Monteverdia ilicifolia
Sci. Rep.
6
36858
2016
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1
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1
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4
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2
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1
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1
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1
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2
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1
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2
2
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715543
Wang
Cloning and characterization o ...
Kalanchoe daigremontiana
J. Biol. Chem.
285
29703-29712
2010
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1
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4
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1
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