Literature summary for 5.4.99.5 extracted from

Sasso, S.; Okvist, M.; Roderer, K.; Gamper, M.; Codoni, G.; Krengel, U.; Kast, P.
Structure and function of a complex between chorismate mutase and DAHP synthase: efficiency boost for the junior partner (2009), EMBO J., 28, 2128-2142.

Search for more literature for 5.4.99.5

Activating Compound

Activating Compound	Comment	Organism	Structure
3-deoxy-D-arabino-heptulosonate-7-phosphate synthase	the catalytic efficiency of chorismate mutase increases 140fold on addition of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, chorismate mutase forms a complex with 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and that complex formation both increases the CM activity by more than two orders of magnitude and endows chorismate mutase with regulatory features	Mycobacterium tuberculosis
additional information	neither pH variation between 5.9 and 8.7, nor provision of 0.1 mg/ml bovine serum albumin, 2 mM Ca2+, 10 mM Mg2+, 1 mM EDTA, 1 mM EGTA, 1 mM 1,10-phenanthroline, 1 mM L-phenylalanine, 1 mM L-tyrosine, 1 mM L-tryptophan, or 0.6 mM salicylate affect catalytic activity by more than a factor of 2	Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain KA13	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with L-malate, hanging drop vapor diffusion method, using 15% (w/v) PEG 1500 and 0.1 M of the L-malate-containing MMT buffer system pH 8.0-9.0	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
G86A	mutant shows reduced kcat and Km values compared to the wild type enzyme	Mycobacterium tuberculosis
K39R	inactive	Mycobacterium tuberculosis
L88A	inactive	Mycobacterium tuberculosis
R46K	inactive	Mycobacterium tuberculosis
R87A	inactive	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
L-Phe	28% residual activity at 0.2 mM	Mycobacterium tuberculosis
L-Trp	96% residual activity at 0.2 mM	Mycobacterium tuberculosis
L-Tyr	63% residual activity at 0.2 mM	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.57	-	chorismate	mutant enzyme G86A	Mycobacterium tuberculosis
1.14	-	chorismate	wild type enzyme	Mycobacterium tuberculosis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Mycobacterium tuberculosis	5622	-

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WIC1	-	-
Mycobacterium tuberculosis H37Rv	P9WIC1	-	-

Purification (Commentary)

Purification (Comment)	Organism
anion exchange column chromatography, Superdex 75 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Chorismate	-	Mycobacterium tuberculosis	Prephenate	-	?
Chorismate	-	Mycobacterium tuberculosis H37Rv	Prephenate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.11	-	chorismate	mutant enzyme G86A	Mycobacterium tuberculosis
2	-	chorismate	wild type enzyme	Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.19	-	chorismate	mutant enzyme G86A	Mycobacterium tuberculosis
1.75	-	chorismate	wild type enzyme	Mycobacterium tuberculosis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)