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Literature summary for 5.4.99.5 extracted from

  • Kim, S.K.; Reddy, S.K.; Nelson, B.C.; Robinson, H.; Reddy, P.T.; Ladner, J.E.
    A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H(37)R(v) and the secreted chorismate mutase (y2828) from Yersinia pestis (2008), FEBS J., 275, 4824-4835.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis
expressed in Escherichia coli BL21(DE3) cells Yersinia pestis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, in 2 M ammonium sulfate, 0.1 M citrate/phosphate buffer, pH 4.2 Yersinia pestis
sitting drop vapour diffusion method, using 0.1 M Tris-HCl (pH 8.6), 0.2 M MgCl2, and 20% poly(ethylene glycol) 400 for the 90 amino acid enzyme 90-MtCM Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
chorismate at 37°C and pH 7.5 Yersinia pestis
1.5
-
chorismate at 37°C and pH 7.5 Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Yersinia pestis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6000
-
2 * 6000, SDS-PAGE Mycobacterium tuberculosis
10090
-
90-MtCM, MALDI-TOF mass spectrometry and calculated from amino acid sequence Mycobacterium tuberculosis
11770
-
105-MtCM, MALDI-TOF mass spectrometry and calculated from amino acid sequence Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WIC1
-
-
Mycobacterium tuberculosis H37Rv P9WIC1
-
-
Yersinia pestis Q7CHH5
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Yersinia pestis
subtilisin column chromatography and Sephadex G-75 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
Mycobacterium tuberculosis Prephenate
-
?
Chorismate
-
Yersinia pestis Prephenate
-
?
Chorismate
-
Mycobacterium tuberculosis H37Rv Prephenate
-
?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Yersinia pestis
homodimer 2 * 6000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
105-MtCM
-
Mycobacterium tuberculosis
90-MtCM
-
Mycobacterium tuberculosis
Rv0948c
-
Mycobacterium tuberculosis
y2828
-
Yersinia pestis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5
-
chorismate at 37°C and pH 7.5 Mycobacterium tuberculosis
70
-
chorismate at 37°C and pH 7.5 Yersinia pestis