Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
expressed in Escherichia coli BL21(DE3) cells | Yersinia pestis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, in 2 M ammonium sulfate, 0.1 M citrate/phosphate buffer, pH 4.2 | Yersinia pestis |
sitting drop vapour diffusion method, using 0.1 M Tris-HCl (pH 8.6), 0.2 M MgCl2, and 20% poly(ethylene glycol) 400 for the 90 amino acid enzyme 90-MtCM | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
chorismate | at 37°C and pH 7.5 | Yersinia pestis | |
1.5 | - |
chorismate | at 37°C and pH 7.5 | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Yersinia pestis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
6000 | - |
2 * 6000, SDS-PAGE | Mycobacterium tuberculosis |
10090 | - |
90-MtCM, MALDI-TOF mass spectrometry and calculated from amino acid sequence | Mycobacterium tuberculosis |
11770 | - |
105-MtCM, MALDI-TOF mass spectrometry and calculated from amino acid sequence | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WIC1 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WIC1 | - |
- |
Yersinia pestis | Q7CHH5 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Yersinia pestis |
subtilisin column chromatography and Sephadex G-75 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | - |
Mycobacterium tuberculosis | Prephenate | - |
? | |
Chorismate | - |
Yersinia pestis | Prephenate | - |
? | |
Chorismate | - |
Mycobacterium tuberculosis H37Rv | Prephenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Yersinia pestis |
homodimer | 2 * 6000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
105-MtCM | - |
Mycobacterium tuberculosis |
90-MtCM | - |
Mycobacterium tuberculosis |
Rv0948c | - |
Mycobacterium tuberculosis |
y2828 | - |
Yersinia pestis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
chorismate | at 37°C and pH 7.5 | Mycobacterium tuberculosis | |
70 | - |
chorismate | at 37°C and pH 7.5 | Yersinia pestis |