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Literature summary for 5.4.99.5 extracted from

  • Kast, P.; Grisostomi, C.; Chen, I.A.; Li, S.; Krengel, U.; Xue, Y.; Hilvert, D.
    A strategically positioned cation is crucial for efficient catalysis by chorismate mutase (2000), J. Biol. Chem., 275, 36832-36838.
    View publication on PubMed
Search for more literature for 5.4.99.5

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of double mutants C88S/R90K and C88K/R90S, hanging drop vapour diffusion method at room temperature, space group R3 with a and b: 82.6 A and c: 42.8 A Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
C88K/R90S lower activity than wild-type enzyme Bacillus subtilis
C88S/R90K lower activity than wild-type enzyme Bacillus subtilis
R90A no activity detectable Bacillus subtilis
R90G very low activity Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
oxabicyclic dicarboxylic acid transition state analogon, competitive inhibition Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
 chorismate 30°C, pH 7, wild-type Bacillus subtilis
0.15
-
 chorismate 30°C, pH 7, mutant R90G Bacillus subtilis
1.9
-
 chorismate 30°C, pH 7, mutant C88K/R90S Bacillus subtilis
4.3
-
 chorismate 30°C, pH 7, mutant C88S/R90K Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Chorismate Bacillus subtilis first committed step in the biosynthesis of the aromatic amino acids tyrosine and phenylalanine in bacteria, fungi and higher plants Prephenate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P19080
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
 Bacillus subtilis Prephenate
-
 ?
Chorismate first committed step in the biosynthesis of the aromatic amino acids tyrosine and phenylalanine in bacteria, fungi and higher plants Bacillus subtilis Prephenate
-
 ?

Synonyms

Synonyms Comment Organism
BsCM
-
 Bacillus subtilis
chorismate mutase
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
 chorismate 30°C, pH 7, mutant R90G Bacillus subtilis
0.29
-
 chorismate 30°C, pH 7, mutant C88S/R90K Bacillus subtilis
0.32
-
 chorismate 30°C, pH 7, mutant C88K/R90S Bacillus subtilis
46
-
 chorismate 30°C, pH 7, wild-type Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 oxabicyclic dicarboxylic acid >> 1 mM mutant C88S/R90K Bacillus subtilis
0.003
-
 oxabicyclic dicarboxylic acid 30°C, pH 7, wild-type Bacillus subtilis
0.004
-
 oxabicyclic dicarboxylic acid 30°C, pH 7, mutant R90G Bacillus subtilis
1.1
-
 oxabicyclic dicarboxylic acid 30°C, pH 7, mutant C88K/R90S Bacillus subtilis