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Literature summary for 5.4.99.5 extracted from
- The aroC gene of Aspergillus nidulans codes for a monofunctional, allosterically regulated chorismate mutase (1999), J. Biol. Chem., 274, 22275-22282.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| tryptophan | 0.005 mM, heterotrophic activator | Aspergillus nidulans |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in a Saccharomyces cerevisiae aro7DELTA mutant strain | Aspergillus nidulans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D233I | little reduced regulatory range through tyrosine and tryptophan than wild-type enzyme | Aspergillus nidulans |
| D233T | strong reduced regulatory range through tyrosine and tryptophan than wild-type enzyme | Aspergillus nidulans |
| T226D | reduced regulatory range through tyrosine and tryptophan than wild-type enzyme | Saccharomyces cerevisiae |
| T226I | reduced regulatory range through tyrosine and tryptophan than wild-type enzyme | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chorismate | - |
Aspergillus nidulans | |
| tyrosine | 0.05 mM, heterotrophic feedback-inhibitor | Aspergillus nidulans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
alpha2, 2 * 30000, calculated from amino acid sequence, homology modeling, electron microscopy | Aspergillus nidulans |
| 62200 | - |
gel filtration | Aspergillus nidulans |
| 65000 | - |
PAGE | Aspergillus nidulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | Saccharomyces cerevisiae | enzyme of the first branch point of aromatic amino acid biosynthesis | Prephenate | - |
? | |
| Chorismate | Aspergillus nidulans | enzyme of the first branch point of aromatic amino acid biosynthesis | Prephenate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | Q9Y7B2 | - |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus nidulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1200 | - |
0.1 mM tyrosine, wild-type | Saccharomyces cerevisiae |
| 3300 | - |
0.1 mM tyrosine, mutant T226D | Saccharomyces cerevisiae |
| 3700 | - |
unliganded, mutant T226D | Saccharomyces cerevisiae |
| 4800 | - |
unliganded, wild-type | Saccharomyces cerevisiae |
| 8400 | - |
0.1 mM tyrosine, wild-type | Aspergillus nidulans |
| 11100 | - |
0.5 mM tryptophan, mutant T226D | Saccharomyces cerevisiae |
| 13400 | - |
0.1 mM tyrosine, mutant D233I | Aspergillus nidulans |
| 20600 | - |
unliganded, mutant T226I | Saccharomyces cerevisiae |
| 21700 | - |
0.1 mM tyrosine, mutant T226I | Saccharomyces cerevisiae |
| 22000 | - |
0.1 mM tyrosine, mutant D233T | Aspergillus nidulans |
| 26400 | - |
0.5 mM tryptophan, mutant T226I | Saccharomyces cerevisiae |
| 30300 | - |
unliganded, mutant D233I | Aspergillus nidulans |
| 32500 | - |
unliganded, wild-type | Aspergillus nidulans |
| 40200 | - |
0.5 mM tryptophan, wild-type | Saccharomyces cerevisiae |
| 41300 | - |
unliganded, mutant D233T | Aspergillus nidulans |
| 63000 | - |
0.5 mM tryptophan, mutant D233I | Aspergillus nidulans |
| 64300 | - |
0.5 mM tryptophan, mutant D233T | Aspergillus nidulans |
| 88500 | - |
0.5 mM tryptophan, wild-type | Aspergillus nidulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | - |
Saccharomyces cerevisiae | Prephenate | - |
? | |
| Chorismate | - |
Aspergillus nidulans | Prephenate | - |
? | |
| Chorismate | enzyme of the first branch point of aromatic amino acid biosynthesis | Saccharomyces cerevisiae | Prephenate | - |
? | |
| Chorismate | enzyme of the first branch point of aromatic amino acid biosynthesis | Aspergillus nidulans | Prephenate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | alpha2, 2 * 30000, calculated from amino acid sequence, homology modeling, electron microscopy | Aspergillus nidulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chorismate mutase | - |
Saccharomyces cerevisiae |
| chorismate mutase | - |
Aspergillus nidulans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 82 | - |
chorismate | 30°C, pH 7.6 | Aspergillus nidulans | |
| 92 | - |
chorismate | 30°C, pH 7.6, in presence of 0.005 mM tryptophan | Aspergillus nidulans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
catalytic maximum in presence of tyrosine | Aspergillus nidulans |
| 7.1 | - |
catalytic maximum in presence of tryptophan | Aspergillus nidulans |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
chorismate | 30°C, pH 7.6, in presence of 0.005 mM tyrosine | Aspergillus nidulans | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus nidulans | isoelectric focusing, pH-gradient 3.5-9.5 | 4.8 | 4.7 |
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