Any feedback?
Literature summary for 5.4.99.5 extracted from
- Chorismate mutase of Thermus thermophilus is a monofunctional AroH class enzyme inhibited by tyrosine (2004), Arch. Microbiol., 181, 195-203.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli with a His-tag | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tyrosine | competitive inhibition, 0.5 M tyrosine leads to 40% inhibition | Thermus thermophilus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.29 | - |
chorismate | 70°C, pH 7.6 | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15800 | - |
alpha3, 3 * 15800, SDS-PAGE | Thermus thermophilus |
| 47590 | - |
gel filtration | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | Thermus thermophilus | biosynthesis of aromatic amino acids | Prephenate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | Q84FH6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25 | - |
after affinity chromatography | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | - |
Thermus thermophilus | Prephenate | - |
? | |
| Chorismate | biosynthesis of aromatic amino acids | Thermus thermophilus | Prephenate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | alpha3, 3 * 15800, SDS-PAGE | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chorismate mutase | - |
Thermus thermophilus |
| TtCM | - |
Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
half-life of 61.7 min | Thermus thermophilus |
| 50 | - |
half-life of 42.7 min | Thermus thermophilus |
| 70 | - |
half-life of 8.1 min | Thermus thermophilus |
| 80 | - |
half-life of 6.5 min | Thermus thermophilus |
| 100 | - |
half-life of 1.4 min | Thermus thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 52 | - |
chorismate | 70°C, pH 7.6 | Thermus thermophilus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
tyrosine | 70°C, pH 7.6 | Thermus thermophilus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
