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Literature summary for 5.4.99.5 extracted from

  • Schnappauf, G.; Lipscomb, W.N.; Braus, G.H.
    Separation of inhibition and activation of the allosteric yeast chorismate mutase (1998), Proc. Natl. Acad. Sci. USA, 95, 2868-2873.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.5

Activating Compound

Activating Compound Comment Organism Structure
L-Trp wild-type enzyme, mutant enzyme Ile225Thr/Thr226Ile and enzymes with mutations at Tyr234 are activated. No activation of mutant enzyme Thr226Ile Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
I225T mutant enzyme Ile225Thr is activated by Trp, but is insensitive to Tyr Saccharomyces cerevisiae
Y234F enzymes with mutations of Tyr234, especially Tyr234Phe are unresponsive to Tyr but are activated by Trp Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
L-Tyr wild-type enzyme is inhibited. Mutant enzymes with amino acid exchange at Thr234, especially Tyr234Phe, mutant enzyme Ile225Thr and Ile225Thr/Thr226Ile are insensitive Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 chorismate chorismate, mutant enzyme Thr226Ile, activated by 0.01 mM Trp Saccharomyces cerevisiae
0.4
-
 chorismate wild-type enzyme, activated by 0.01 mM Trp Saccharomyces cerevisiae
0.7
-
 chorismate mutant enzyme Tyr234Phe, activated by 0.01 mM Trp Saccharomyces cerevisiae
0.8
-
 chorismate mutant enzyme Tyr234Ala, activated by 0.01 mM Trp Saccharomyces cerevisiae
1.3
-
 chorismate mutant enzyme Tyr234Ser, activated by 0.01 mM Trp Saccharomyces cerevisiae
1.5
-
 chorismate mutant enzyme Ile225Thr/Thr226Ile, activated by 0.01 mM Trp Saccharomyces cerevisiae
1.6
-
 chorismate mutant enzyme Glu23Asp, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.2
-
 chorismate mutant enzyme Glu23Ala, activated by 0.01 mM Trp Saccharomyces cerevisiae
6
-
 chorismate mutant enzyme Glu23Gln, activated by 0.01 mM Trp Saccharomyces cerevisiae
6.3
-
 chorismate mutant enzyme Tyr234Glu, activated by 0.01 mM Trp Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
 Saccharomyces cerevisiae Prephenate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
 chorismate mutant enzyme Glu23Ala, activated by 0.01 mM Trp Saccharomyces cerevisiae
44
-
 chorismate mutant enzyme Tyr234Glu, activated by 0.01 mM Trp Saccharomyces cerevisiae
74
-
 chorismate mutant enzyme Tyr234Ser, activated by 0.01 mM Trp Saccharomyces cerevisiae
171
-
 chorismate mutant enzyme Glu23Gln, activated by 0.01 mM Trp Saccharomyces cerevisiae
252
-
 chorismate mutant enzyme Tyr234Ala, activated by 0.01 mM Trp Saccharomyces cerevisiae
351
-
 chorismate mutant enzyme Thr226Ile, activated by 0.01 mM Trp Saccharomyces cerevisiae
361
-
 chorismate wild-type enzyme, activated by 0.01 mM Trp Saccharomyces cerevisiae
535
-
 chorismate mutant enzyme Ile225Thr/Thr226Ile, activated by 0.01 mM Trp Saccharomyces cerevisiae
565
-
 chorismate mutant enzyme Tyr234Phe, activated by 0.01 mM Trp Saccharomyces cerevisiae
625
-
 chorismate mutant enzyme Glu23Asp, activated by 0.01 mM Trp Saccharomyces cerevisiae