Literature summary for 5.4.99.47 extracted from

Khenkin, A.; Neumann, R.
Oxidosqualene Cyclase Residues that Promote Formation of Cycloartenol, Lanosterol, and Parkeol We are grateful to Bridget M. Joubert for advice regarding mutagenesis. We thank Elizabeth A. Hart for an authentic parkeol standard, and for chromatographic and spectroscopic information. This research was funded by the National Institutes of Health (grant no. AI 41598) and the Robert A. Welch Foundation (grant no. C-1323). M.M.M. was an American Society of Pharmacognosy Undergraduate Fellow. M.J.R.S. was a Robert A. Welch Fellow and was supported by an NIH Biotechnology Training Grant (grant no. T32 GM08362). (2000), Angew. Chem., 39, 4090-4092.

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(S)-2,3-oxidosqualene	-	Saccharomyces cerevisiae	parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol	lanosterol-synthase mutant T384Y produces 11% parkeol, 79% lanosterol and 10% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene	?
(S)-2,3-oxidosqualene	-	Saccharomyces cerevisiae	parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol	lanosterol-synthase mutant T384Y/V4545L produces 60% parkeol, 20% lanosterol and 20% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene	?
(S)-2,3-oxidosqualene	-	Saccharomyces cerevisiae	parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol	lanosterol-synthase mutant T384Y/V454I produces 64% parkeol, 13% lanosterol and 23% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene	?

Synonyms	Comment	Organism
lanosterol-synthase mutant T384Y	cf. EC 5.4.99.7	Saccharomyces cerevisiae
lanosterol-synthase mutant T384Y/V454I	cf. EC 5.4.99.7	Saccharomyces cerevisiae
lanosterol-synthase mutant T384Y/V454L	cf. EC 5.4.99.7	Saccharomyces cerevisiae