BRENDA - Enzyme Database show
show all sequences of 5.4.99.46

Molecular characterization of an oxidosqualene cyclase that yields shionone, a unique tetracyclic triterpene ketone of Aster tataricus

Sawai, S.; Uchiyama, H.; Mizuno, S.; Aoki, T.; Akashi, T.; Ayabe, S.; Takahashi, T.; FEBS Lett. 585, 1031-1036 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in lanosterol synthase-deficient yeast; expressed in Saccharomyces cerevisiae strain GIL77 (lanosterol synthase-deficient strain)
Aster tataricus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aster tataricus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
; high expression
Aster tataricus
-
leaf
; high expression
Aster tataricus
-
radical leaf
-
Aster tataricus
-
rhizome
-
Aster tataricus
-
root
-
Aster tataricus
-
stem
; high expression
Aster tataricus
-
stolon
-
Aster tataricus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
715030
Aster tataricus
shionone + beta-amyrin + friedelin + dammara-20,24-dienol + 4-epishionone
the ratio of the products is roughly estimated as shionone:beta-amyrin:friedelin:dammara-20,24-dienol:4-epishionone = 90%:2%:2%:1.5%:1.5%
-
-
?
oxidosqualene
-
715030
Aster tataricus
shionone
the cyclase yields ca. 90% shionone and small amounts of beta-amyrin, friedelin, dammara-20,24-dienol, and 4-epishionone
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in lanosterol synthase-deficient yeast; expressed in Saccharomyces cerevisiae strain GIL77 (lanosterol synthase-deficient strain)
Aster tataricus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
; high expression
Aster tataricus
-
leaf
; high expression
Aster tataricus
-
radical leaf
-
Aster tataricus
-
rhizome
-
Aster tataricus
-
root
-
Aster tataricus
-
stem
; high expression
Aster tataricus
-
stolon
-
Aster tataricus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
715030
Aster tataricus
shionone + beta-amyrin + friedelin + dammara-20,24-dienol + 4-epishionone
the ratio of the products is roughly estimated as shionone:beta-amyrin:friedelin:dammara-20,24-dienol:4-epishionone = 90%:2%:2%:1.5%:1.5%
-
-
?
oxidosqualene
-
715030
Aster tataricus
shionone
the cyclase yields ca. 90% shionone and small amounts of beta-amyrin, friedelin, dammara-20,24-dienol, and 4-epishionone
-
-
?
Other publictions for EC 5.4.99.46
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
715030
Sawai
Molecular characterization of ...
Aster tataricus
FEBS Lett.
585
1031-1036
2011
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