Literature summary for 5.4.99.41 extracted from

Kushiro, T.; Shibuya, M.; Masuda, K.; Ebizuka, Y.
Mutational studies on triterpene synthases: engineering lupeol synthase into beta-amyrin synthase (2000), J. Am. Chem. Soc., 122, 6816-6824.

No PubMed abstract available

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Protein Variants

Protein Variants	Comment	Organism
L256A	mutation in region responsible for product differentiation. Mutant produces 3.8% beta-amyrin, 69.5% lupeol, 10% butyrospermol and 13.5% of dammara-18(E),21-dien-3beta-ol, dammara-18(Z),21-dien-3beta-ol and dammara-18(28),21-dien-3beta-ol	Olea europaea
L256F	mutation in region responsible for product differentiation. Mutant produces 9.8% beta-amyrin, 69.7% lupeol, 17.9% butyrospermol	Olea europaea
L256H	mutation in region responsible for product differentiation. Mutant produces 3.8% beta-amyrin, 69.5% lupeol, 10% butyrospermol and 13.5% of dammara-18(E),21-dien-3beta-ol, dammara-18(Z),21-dien-3beta-ol and dammara-18(28),21-dien-3beta-ol	Olea europaea
L256W	mutation in region responsible for product differentiation. Mutant produces 74.8% beta-amyrin, 6.9% lupeol, 9.9% butyrospermol and 8.4% germanicol	Olea europaea
L256Y	mutation in region responsible for product differentiation. Mutant produces 1.6% beta-amyrin, 54.8% lupeol, 22.7% butyrospermol and 18.7% of dammara-18(E),21-dien-3beta-ol, dammara-18(Z),21-dien-3beta-ol and dammara-18(28),21-dien-3beta-ol	Olea europaea
Y258H	mutation in region responsible for product differentiation. Mutant produces 43.6% lupeol, 6.2% butyrospermol and 42.4% of dammara-18(E),21-dien-3beta-ol, dammara-18(Z),21-dien-3beta-ol and dammara-18(28),21-dien-3beta-ol	Olea europaea

Organism

Organism	UniProt	Comment	Textmining
Olea europaea	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,3-oxidosqualene	-	Olea europaea	lupeol	-	?

Synonyms

Synonyms	Comment	Organism
OEW	-	Olea europaea

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Release 2023.1 (January 2023)