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Literature summary for 5.4.99.4 extracted from

  • Barker, H.A.
    Coenzyme B12-dependent mutases causing carbon chain rearrangements (1972), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 6, 509-537.
No PubMed abstract available
Search for more literature for 5.4.99.4

Inhibitors

Inhibitors Comment Organism Structure
1-Methyl-1,2-cis-cyclopropanedicarboxylate noncompetitive, slight inhibitor Eubacterium barkeri
1-Methyl-1,2-trans-cyclopropanedicarboxylate competitive Eubacterium barkeri
glutaconate noncompetitive Eubacterium barkeri
Itaconate competitive Eubacterium barkeri
L-malate competitive Eubacterium barkeri
mesaconate competitive Eubacterium barkeri
succinate competitive Eubacterium barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1
-
 2-Methyleneglutarate
-
 Eubacterium barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 sucrose density gradient centrifugation Eubacterium barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Eubacterium barkeri inducible enzyme, participates in the anaerobic conversion of nicotinic acid to equimolar amounts of propionate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Eubacterium barkeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Eubacterium barkeri

Reaction

Reaction Comment Organism Reaction ID
2-Methyleneglutarate = 2-methylene-3-methylsuccinate mechanism Eubacterium barkeri
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.51
-
 preparation which is heavily contaminated with methylitaconate isomerase Eubacterium barkeri

Storage Stability

Storage Stability Organism
-80°C, protein concentration 10 mg/ml, about 10% loss of activity after 6 months Eubacterium barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Methyleneglutarate r Eubacterium barkeri 2-Methylene-3-methylsuccinate i.e methylitaconate ?
additional information inducible enzyme, participates in the anaerobic conversion of nicotinic acid to equimolar amounts of propionate Eubacterium barkeri ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
cobamide required Eubacterium barkeri
vitamin B12 dependent on Eubacterium barkeri