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Literature summary for 5.4.99.39 extracted from
- beta-Amyrin synthase (EsBAS) and beta-amyrin 28-oxidase (CYP716A244) in oleanane-type triterpene saponin biosynthesis in Eleutherococcus senticosus (2017), Phytochemistry, 135, 53-63 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene EsBAS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, transcriptomics analysis of plant leaves, quantitative PCR enzyme expression analysis, recombinant expression of teh enzyme in Saccharomyces cerevisiae ERG7-deficient mutant strain. Functional recombinant coexpression of the gene encoding hygromycin phosphotransferase (HPT), gene CYP716A244 (EC 1.14.14.126), and gene EsBAS in transgenic tobacco plant leaves via Agrobacterium tumefaciens strain GV3101-mediated transformation. Metabolite identification by GC/MS analysis | Eleutherococcus senticosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-2,3-epoxy-2,3-dihydrosqualene | Eleutherococcus senticosus | - |
beta-amyrin | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eleutherococcus senticosus | A0A1P8W708 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bark | - |
Eleutherococcus senticosus | - |
| leaf | low expression level | Eleutherococcus senticosus | - |
| additional information | transcriptomics analysis of plant leaves, quantitative order of EsBAS mRNA accumulation is roots - stem barks - leaves in descending order | Eleutherococcus senticosus | - |
| root | highest expression level | Eleutherococcus senticosus | - |
| stem | - |
Eleutherococcus senticosus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Eleutherococcus senticosus | beta-amyrin | - |
r | |
| additional information | metabolite identification by GC/MS analysis | Eleutherococcus senticosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 88400, about, sequence calculation | Eleutherococcus senticosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EsBAS | - |
Eleutherococcus senticosus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Eleutherococcus senticosus | the enzyme is induced by methyl jasmonate (MeJA). The transcription of EsBAS is clearly enhanced at 12 and 24 h after MeJA treatment and reduced to the level of the control (without MeJA treatment) at 72 h after MeJA treatment | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the putative saponin biosynthetic pathway from 2,3-oxidosqualene in Eleutherococcus senticosus, overview | Eleutherococcus senticosus |
| physiological function | beta-amyrin in Eleutherococcus senticosus may play a key role in saponin biosynthesis | Eleutherococcus senticosus |
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Release 2023.1 (January 2023)
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