Literature summary for 5.4.99.39 extracted from

Kushiro, T.; Shibuya, M.; Masuda, K.; Ebizuka, Y.
Mutational studies on triterpene synthases: engineering lupeol synthase into beta-amyrin synthase (2000), J. Am. Chem. Soc., 122, 6816-6824.

No PubMed abstract available

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Protein Variants

Protein Variants	Comment	Organism
C262S	mutation in region responsible for product differentiation. Like wild-type, mutant produces beta-amyrin as sole product	Panax ginseng
M258I/W259L	mutation in region responsible for product differentiation. Mutant produces 40.5% beta-amyrin, 53.4% lupeol, 3.6% butyrospermol and 2.5% germanicol	Panax ginseng
W259L	mutation in region responsible for product differentiation. Mutant produces 30.3% beta-amyrin, 54.6% lupeol, 3.6% butyrospermol and 3.4% germanicol	Panax ginseng
Y261H	mutation in region responsible for product differentiation. Mutant produces 2.4% lupeol, 13.6% germanicol and 84% of dammara-18(E),21-dien-3beta-ol, dammara-18(Z),21-dien-3beta-ol and dammara-18(28),21-dien-3beta-ol	Panax ginseng

Organism

Organism	UniProt	Comment	Textmining
Panax ginseng	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,3-oxidosqualene	-	Panax ginseng	beta-amyrin	-	?

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Release 2023.1 (January 2023)