BRENDA - Enzyme Database show
show all sequences of 5.4.99.34

Multifunctional oxidosqualene cyclases and cytochrome P450 involved in the biosynthesis of apple fruit triterpenic acids

Andre, C.; Legay, S.; Deleruelle, A.; Nieuwenhuizen, N.; Punter, M.; Brendolise, C.; Cooney, J.; Lateur, M.; Hausman, J.; Larondelle, Y.; Laing, W.; New Phytol. 211, 1279-1294 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene OSC4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of germanicol, beta-amyrin, and lupeol
Malus domestica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
Malus domestica
-
germanicol
-
-
r
additional information
Malus domestica
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Malus domestica
A0A142LT03
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Malus domestica
-
additional information
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
Malus domestica
-
plant epidermis
-
Malus domestica
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
748772
Malus domestica
germanicol
-
-
-
r
(S)-2,3-oxidosqualene
via dammarenyl cation, low activity
748772
Malus domestica
beta-amyrin
-
-
-
r
(S)-2,3-oxidosqualene
via dammarenyl cation, low activity
748772
Malus domestica
lupeol
-
-
-
r
additional information
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
748772
Malus domestica
?
-
-
-
-
additional information
gene MdOSC4 encodes a multifunctional oxidosqualene cyclase producing an oleanane-type triterpene, putatively identified as germanicol, as well as beta-amyrin and lupeol, in the proportion 82:14:4
748772
Malus domestica
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
gene OSC4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of germanicol, beta-amyrin, and lupeol
Malus domestica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
Malus domestica
-
germanicol
-
-
r
additional information
Malus domestica
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Malus domestica
-
additional information
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
Malus domestica
-
plant epidermis
-
Malus domestica
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
748772
Malus domestica
germanicol
-
-
-
r
(S)-2,3-oxidosqualene
via dammarenyl cation, low activity
748772
Malus domestica
beta-amyrin
-
-
-
r
(S)-2,3-oxidosqualene
via dammarenyl cation, low activity
748772
Malus domestica
lupeol
-
-
-
r
additional information
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
748772
Malus domestica
?
-
-
-
-
additional information
gene MdOSC4 encodes a multifunctional oxidosqualene cyclase producing an oleanane-type triterpene, putatively identified as germanicol, as well as beta-amyrin and lupeol, in the proportion 82:14:4
748772
Malus domestica
?
-
-
-
-
General Information
General Information
Commentary
Organism
evolution
MdOSC4 contains the highly conserved DCTAE motif, which is implicated in substrate binding, and six repeats of the QW motifs, typical of the triterpene synthase superfamily, sequence comparisons and phylogenetic analysis
Malus domestica
metabolism
analysis of biosynthetic pathway of triterpenic acids in apple fruits, overview
Malus domestica
additional information
analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview
Malus domestica
physiological function
the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar
Malus domestica
General Information (protein specific)
General Information
Commentary
Organism
evolution
MdOSC4 contains the highly conserved DCTAE motif, which is implicated in substrate binding, and six repeats of the QW motifs, typical of the triterpene synthase superfamily, sequence comparisons and phylogenetic analysis
Malus domestica
metabolism
analysis of biosynthetic pathway of triterpenic acids in apple fruits, overview
Malus domestica
additional information
analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview
Malus domestica
physiological function
the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar
Malus domestica
Other publictions for EC 5.4.99.34
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748772
Andre
Multifunctional oxidosqualene ...
Malus domestica
New Phytol.
211
1279-1294
2016
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5
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4
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683462
Basyuni
Triterpene synthases from the ...
Rhizophora stylosa
FEBS J.
274
5028-5042
2007
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