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Literature summary for 5.4.99.33 extracted from

  • Dai, L.; Liu, C.; Zhu, Y.; Zhang, J.; Men, Y.; Zeng, Y.; Sun, Y.
    Functional characterization of cucurbitadienol synthase and triterpene glycosyltransferase involved in biosynthesis of mogrosides from Siraitia grosvenorii (2015), Plant Cell Physiol., 56, 1172-1182 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene CBS, DNA and amino acid sequence determination and analysis, digital gene expression (DGE) profile analysis, sequence comparisons and phylogenetic analysis of codon-optimized gene, SgCbQ is functionally expressed in the lanosterol synthase-deficient Saccharomyces cerevisiae strain GIL77 leading to accumulation of cucurbitadienol as the sole product Siraitia grosvenorii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene Siraitia grosvenorii
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 cucurbitadienol
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 ?
additional information Siraitia grosvenorii GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol ?
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 ?

Organism

Organism UniProt Comment Textmining
Siraitia grosvenorii K7NBZ9 cultivated in Guangxi Province of China
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Source Tissue

Source Tissue Comment Organism Textmining
fruit expression profiles of SgCbQ gene during the three important stages of fruit development Siraitia grosvenorii
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene
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 Siraitia grosvenorii cucurbitadienol
-
 ?
additional information GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol Siraitia grosvenorii ?
-
 ?

Synonyms

Synonyms Comment Organism
CBS
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 Siraitia grosvenorii
SgCbQ
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 Siraitia grosvenorii

General Information

General Information Comment Organism
evolution SgCbQ clusters with the other three functionally characterized cucurbitadienol synthases and forms a new subfamily that lies between the branches of cycloartenol synthase and lanosterol synthase, non-rooted phylogenetic tree analysis, overview Siraitia grosvenorii
metabolism cucurbitadienol synthase and triterpene glycosyltransferase are involved in biosynthesis of mogrosides in Siraitia grosvenorii. Mogrosides, the major bioactive components isolated from the fruits of Siraitia grosvenorii, are a family of cucurbitane-type tetracyclic triterpenoid saponins that are used worldwide as high-potency sweeteners and possess a variety of notable pharmacological activities. Mogrosides are synthesized from 2,3-oxidosqualene via a series of reactions catalyzed by cucurbitadienol synthase (CbQ), Cyt P450s (P450s), and UDP glycosyltransferases (UGTs) in vivo, proposed biosynthetic pathway of mogrosides, overview Siraitia grosvenorii