Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.30 extracted from

  • Honta, H.; Inamura, T.; Konishi, T.; Satoh, S.; Iwai, H.
    UDP-arabinopyranose mutase gene expressions are required for the biosynthesis of the arabinose side chain of both pectin and arabinoxyloglucan, and normal leaf expansion in Nicotiana tabacum (2018), J. Plant Res., 131, 307-317 .
    View publication on PubMed
Search for more literature for 5.4.99.30

Cloned(Commentary)

Cloned (Comment) Organism
genes UAM1-4, sequence comparisons and phylogenetic tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression in of RNAi constructs for all 4 isozymes UAM1-4 in Nicotiana tabacum via Agrobacterium tumefaciens strain GV2260-mediated transformation, RT-PCR expression analysis Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
additional information generation of RNAi transformants (NtUAM-KD) to downregulate all four of the UAM members, phenotype, genetic RNAi constructs, overview. Downregulation of NtUAMs expression effects on the localization of arabinan in leaf, and NtUAM knockdown causes the abnormal callus and hole in epidermal tissues Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Nicotiana tabacum 5618
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-beta-L-arabinofuranose Nicotiana tabacum
-
 UDP-beta-L-arabinopyranose
-
 ?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
 cv. Bright Yellow-2
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-beta-L-arabinofuranose
-
 Nicotiana tabacum UDP-beta-L-arabinopyranose
-
 ?

Synonyms

Synonyms Comment Organism
NtUAM
-
 Nicotiana tabacum

General Information

General Information Comment Organism
evolution the Nicotiana tabacum UAM gene family consists of four members Nicotiana tabacum
malfunction the NtUAMKD mutant, with all four isozymes knocked out, shows abnormal leaf development in the form of a callus-like structure and many holes in the leaf epidermis. A clear reduction in the pectic arabinan content is observed in the tissue of the NtUAM-KD leaf. The arabinose/xylose ratio in the xyloglucan-rich cell wall fraction is drastically reduced in NtUAM-KD Nicotiana tabacum
physiological function UDP-arabinopyranose mutase gene expressions are required for the biosynthesis of the arabinose side chain of both pectin and arabinoxyloglucan, and normal leaf expansion in Nicotiana tabacum Nicotiana tabacum