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Literature summary for 5.4.99.30 extracted from
- Analysis of plant UDP-arabinopyranose mutase (UAM) Role of divalent metals and structure prediction (2017), Biochim. Biophys. Acta, 1865, 510-519 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene RGP1, recombinant overexpression of His-MBP-tagged isozyme in Escherichia coli strain BL21 Gold | Arabidopsis thaliana |
| gene RGP2, recombinant overexpression of His-MBP-tagged isozyme in Escherichia coli strain BL21 Gold | Arabidopsis thaliana |
| gene RGP3, recombinant overexpression of His-MBP-tagged isozyme in Escherichia coli strain BL21 Gold | Arabidopsis thaliana |
| isozyme UAM1, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 | Oryza sativa Japonica Group |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activates, best divalent metal ion, optimal at 0.08 mM, about 20% of maximal activity at 1 mM, complete inhibition at 5 mM; activates, best divalent metal ion, optimal at 0.08 mM, about 20% of maximal activity at 1 mM, complete inhibition at 5 mM; activates, best divalent metal ion, optimal at 0.08 mM, about 25% of maximal activity at 1 mM, complete inhibition at 5 mM | Arabidopsis thaliana |  |
| n-butanol | - |
Arabidopsis thaliana | |
| n-butanol | - |
Oryza sativa Japonica Group | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | plant cell wall and leaf cell wall | Arabidopsis thaliana | 5618 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Ca2+ | Arabidopsis thaliana | |
| Ca2+ | displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Ca2+ | Oryza sativa Japonica Group | |
| Ca2+ | displays less than 5% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Ca2+ | Arabidopsis thaliana | |
| Co2+ | displays about 60% of the activity with Mn2+ at 0.08 mM | Arabidopsis thaliana | |
| Co2+ | displays about 60% of the activity with Mn2+ at 0.08 mM | Oryza sativa Japonica Group | |
| Co2+ | displays about 65% of the activity with Mn2+ at 0.08 mM | Arabidopsis thaliana | |
| Cu2+ | displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Cu2+ | Arabidopsis thaliana | |
| Cu2+ | displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Cu2+ | Oryza sativa Japonica Group | |
| Cu2+ | displays less than 5% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Cu2+ | Arabidopsis thaliana | |
| Mg2+ | displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Mg2+ | Arabidopsis thaliana | |
| Mg2+ | displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Mg2+ | Oryza sativa Japonica Group | |
| Mg2+ | displays less than 5% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Mg2+ | Arabidopsis thaliana | |
| Mn2+ | activates, best divalent metal ion, optimal at 0.08 mM, 45% of maximal activity at 5 mM | Oryza sativa Japonica Group | |
| Mn2+ | activates, best divalent metal ion, optimal at 0.08 mM, about 20% of maximal activity at 1 mM, complete inhibition at 5 mM | Arabidopsis thaliana | |
| Mn2+ | activates, best divalent metal ion, optimal at 0.08 mM, about 25% of maximal activity at 1 mM, complete inhibition at 5 mM | Arabidopsis thaliana | |
| additional information | the enzyme requires divalent metal ions for catalytic activity, divalent metal ion dependency of UAM from Arabidopsis thaliana, compared to Oryza sativa | Arabidopsis thaliana | |
| additional information | the enzyme requires divalent metal ions for catalytic activity, divalent metal ion dependency of UAM from Oryza sativa, compared to Arabidopsis thaliana | Oryza sativa Japonica Group | |
| Zn2+ | displays about 60% of the activity with Mn2+ at 0.08 mM | Arabidopsis thaliana | |
| Zn2+ | displays about 70% of the activity with Mn2+ at 0.08 mM | Arabidopsis thaliana | |
| Zn2+ | displays about 70% of the activity with Mn2+ at 0.08 mM | Oryza sativa Japonica Group | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-beta-L-arabinofuranose | Arabidopsis thaliana | - |
UDP-beta-L-arabinopyranose | - |
? | |
| UDP-beta-L-arabinofuranose | Oryza sativa Japonica Group | - |
UDP-beta-L-arabinopyranose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | O22666 | - |
- |
| Arabidopsis thaliana | Q9LFW1 | - |
- |
| Arabidopsis thaliana | Q9SRT9 | - |
- |
| Oryza sativa Japonica Group | Q8H8T0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-MBP-tagged isozyme RGP1 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration | Arabidopsis thaliana |
| recombinant His-MBP-tagged isozyme RGP2 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration | Arabidopsis thaliana |
| recombinant His-MBP-tagged isozyme RGP3 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration | Arabidopsis thaliana |
| recombinant His-tagged wild-type and mutant isozymes UAM1 from Escherichia coli strain BL21 by nickel affinity chromatography | Oryza sativa Japonica Group |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
| additional information | some of the AtRGPs such as the more temperature stable AtRGP2 and tRGP3 may be expressed by the plant as a response to heat stress, or during different stages of development | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-beta-L-arabinofuranose | - |
Arabidopsis thaliana | UDP-beta-L-arabinopyranose | - |
? | |
| UDP-beta-L-arabinofuranose | - |
Oryza sativa Japonica Group | UDP-beta-L-arabinopyranose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtRGP1 | - |
Arabidopsis thaliana |
| AtRGP2 | - |
Arabidopsis thaliana |
| AtRGP3 | - |
Arabidopsis thaliana |
| OsUAM1 | - |
Oryza sativa Japonica Group |
| RGP1 | - |
Arabidopsis thaliana |
| RGP2 | - |
Arabidopsis thaliana |
| RGP3 | - |
Arabidopsis thaliana |
| UAM | - |
Oryza sativa Japonica Group |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Oryza sativa Japonica Group |
| 55 | - |
broad optimum | Arabidopsis thaliana |
| 60 | - |
- |
Arabidopsis thaliana |
| 60 | - |
broad optimum | Arabidopsis thaliana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 100 | over 90% of maximal activity at 40-90°C, 60% at 20°C and 100°C, profile overview | Arabidopsis thaliana |
| 20 | 90 | over 50% of maximal activity within this range, profile overview | Arabidopsis thaliana |
| 30 | 95 | over 80% of maximal activity within this range, 40% at 15°C, over 90% at 35-85°C, profile overview | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Arabidopsis thaliana |
| 6.8 | - |
assay at | Oryza sativa Japonica Group |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | activity range, profile overview | Arabidopsis thaliana |
| 4.5 | 9 | activity range, profile overview | Arabidopsis thaliana |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Arabidopsis thaliana | some of the AtRGPs such as the more temperature stable AtRGP2 and tRGP3 may be expressed by the plant as a response to heat stress, or during different stages of development | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | three-dimensional structure modeling of isozyme UAM1 | Oryza sativa Japonica Group |
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Release 2023.1 (January 2023)
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