Literature summary for 5.4.99.30 extracted from

Konishi, T.; Takeda, T.; Miyazaki, Y.; Ohnishi-Kameyama, M.; Hayashi, T.; O'Neill, M.A.; Ishii, T.
A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose (2006), Glycobiology, 17, 345-354.

Search for more literature for 5.4.99.30

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Oryza sativa

General Stability

General Stability	Organism
repeated cycles of freezing and thawing did not significantly affect mutase activity	Oryza sativa

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	5 mM, complete inhibition; 5 mM, complete inhibition	Oryza sativa
additional information	no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM; no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM	Oryza sativa
UDP-Gal	decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose; decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose	Oryza sativa
UDP-Glc	decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose; decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose	Oryza sativa
UDP-Xyl	decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose; decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose	Oryza sativa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0531	-	UDP-beta-L-arabinofuranose	pH 6.8, 25°C	Oryza sativa
0.055	-	UDP-beta-L-arabinopyranose	pH 6.8, 25°C	Oryza sativa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Oryza sativa	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	5 mM, activity almost doubles	Oryza sativa
additional information	no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM	Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41000	-	x * 41000, SDS-PAGE	Oryza sativa
460000	-	mutase is likely to exist as a complex composed of numerous proteins, gel filtration	Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-beta-L-arabinofuranose	Oryza sativa	-	UDP-beta-L-arabinopyranose	-	r

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	O82706	fragment; L. cv. Koshihikari	-
Oryza sativa	Q8H8T0	L. cv. Koshihikari	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	rUAM1 is autoglycosylated when each protein reacts separately with UDP-Glc. Reversibly glycosylated in the presence of UDP-Glc	Oryza sativa
glycoprotein	rUAM3 is autoglycosylated when each protein reacts separately with UDP-Glc. Reversibly glycosylated in the presence of UDP-Glc	Oryza sativa

Purification (Commentary)

Purification (Comment)	Organism
-	Oryza sativa

Reaction

Reaction	Comment	Organism	Reaction ID
UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose	the reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10)	Oryza sativa	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
seedling	-	Oryza sativa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
35.2	-	-	Oryza sativa

Storage Stability

Storage Stability	Organism
-20°C, mutase activity is stable for at least 3 months	Oryza sativa
4°C, mutase activity is stable for 3 weeks	Oryza sativa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-beta-L-arabinofuranose	-	Oryza sativa	UDP-beta-L-arabinopyranose	-	r
UDP-beta-L-arabinofuranose	the reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10)	Oryza sativa	UDP-beta-L-arabinopyranose	-	r
UDP-beta-L-arabinofuranose	the reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10)	Oryza sativa	UDP-beta-L-arabinopyranose	-	?
UDP-beta-L-arabinopyranose	-	Oryza sativa	UDP-beta-L-arabinofuranose	-	r

Subunits

Subunits	Comment	Organism
?	x * 41000, SDS-PAGE	Oryza sativa

Synonyms

Synonyms	Comment	Organism
Os03g40270 protein	-	Oryza sativa
Os07g41360 protein	-	Oryza sativa
RGP1	-	Oryza sativa
RGPs3	-	Oryza sativa
UAM1	-	Oryza sativa
UAMs3	-	Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Oryza sativa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	6	formation of beta-L-arabinofuranose	Oryza sativa
7	7.5	formation of beta-L-arabinopyranose	Oryza sativa

Cofactor

Cofactor	Comment	Organism	Structure
additional information	the enzyme does not require a cofactor	Oryza sativa

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Release 2023.1 (January 2023)