Literature summary for 5.4.99.29 extracted from

Raychaudhuri, S.; Niu, L.; Conrad, J.; Lane, B.G.; Ofengand, J.
Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA (1999), J. Biol. Chem., 274, 18880-18886.

Search for more literature for 5.4.99.29

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D64N	mutation blocks synthesis of pseudouridine746, mutant rluA constructs produces a stable protein	Escherichia coli
D64T	mutation blocks synthesis of pseudouridine746, mutant rluA constructs produces a stable protein	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
23S rRNA uridine746	Escherichia coli	RluA is the sole synthase for formation of pseudouridine746 in 23S rRNA	23S rRNA pseudouridine746	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AA37	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
23S rRNA uridine746	RluA is the sole synthase for formation of pseudouridine746 in 23S rRNA	Escherichia coli	23S rRNA pseudouridine746	-	?
23S rRNA uridine746	RluA is the sole protein responsible for the in vivo formation of 23S rRNA pseudouridine746	Escherichia coli	23S rRNA pseudouridine746	-	?

Synonyms

Synonyms	Comment	Organism
RluA	-	Escherichia coli

General Information

General Information	Comment	Organism
malfunction	the rluA deletion mutant fails to form either 23S RNA pseudouridine746 or tRNA pseudouridine32. Replacement of rluA in trans on a rescue plasmid restores both pseudouridines. There is no difference in exponential growth rate between wild-type and MG1655(rluA-) either in rich or minimal medium at 24, 37, or 42°C, but when both strains are grown together, a strong selection against the deletion strain is observed	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)