Literature summary for 5.4.99.28 extracted from

Spedaliere, C.J.; Hamilton, C.S.; Mueller, E.G.
Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues (2000), Biochemistry, 39, 9459-9465.

Search for more literature for 5.4.99.28

Protein Variants

Protein Variants	Comment	Organism
P29G	kcat-value is 1.6fold lower than wild-type value. KM-value is 2.9fold higher than wild-type value	Escherichia coli
P29L	kcat-value is 1.9fold lower than wild-type value. KM-value is 4.2fold higher than wild-type value	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000108	-	tRNA uridine32	pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme	Escherichia coli
0.000318	-	tRNA uridine32	pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29G	Escherichia coli
0.000455	-	tRNA uridine32	pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29L	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AA37	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
tRNA uridine32	-	Escherichia coli	tRNA pseudouridine32	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
35	-	Tm-value for mutant enzyme P29L	Escherichia coli
38	-	Tm-value for mutant enzyme P29G is 37.9°C	Escherichia coli
45	-	Tm-value for wild-type enzyme is 44.9°C	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.051	-	tRNA uridine32	pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29L	Escherichia coli
0.061	-	tRNA uridine32	pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29G	Escherichia coli
0.099	-	tRNA uridine32	pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
112.1	-	tRNA uridine32	pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29L	Escherichia coli
191.8	-	tRNA uridine32	pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29G	Escherichia coli
916.7	-	tRNA uridine32	pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme	Escherichia coli

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Release 2023.1 (January 2023)