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Literature summary for 5.4.99.28 extracted from

  • Ramamurthy, V.; Swann, S.; Spedaliere, C.; Mueller, E.
    Role of cysteine residues in pseudouridine synhases of different families (1999), Biochemistry, 38, 13106-13111.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
C117A kcat is 1.4fold lower than wild-type value, KM is 3.9fold higher than wild-type value Escherichia coli
C117A/A128A kcat is 1.3fold lower than wild-type value, KM is 1.5fold higher than wild-type value Escherichia coli
C128A kcat is 1.5fold higher than wild-type value, KM is 1.5fold higher than wild-type value Escherichia coli
additional information substitution of cysteine for amino acids with nonnucleophilic side chains does not significantly alter the catalytic activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000108
-
tRNA uridine32 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme Escherichia coli
0.000157
-
tRNA uridine32 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A/C128A Escherichia coli
0.00016
-
tRNA uridine32 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C128A Escherichia coli
0.00042
-
tRNA uridine32 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AA37
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
tRNA uridine32 = tRNA pseudouridine32 RluA does not require thiol(ate) groups to effect catalysis Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine32 substrate: Escherichia coli tRNAPhe Escherichia coli tRNA pseudouridine32
-
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Synonyms

Synonyms Comment Organism
RluA
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
tRNA uridine32 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A Escherichia coli
0.074
-
tRNA uridine32 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A/C128A Escherichia coli
0.099
-
tRNA uridine32 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme Escherichia coli
0.15
-
tRNA uridine32 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C128A Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
166.7
-
tRNA uridine32 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A Escherichia coli
471.3
-
tRNA uridine32 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A/C128A Escherichia coli
916.7
-
tRNA uridine32 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme Escherichia coli
937.5
-
tRNA uridine32 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C128A Escherichia coli