Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C117A | kcat is 1.4fold lower than wild-type value, KM is 3.9fold higher than wild-type value | Escherichia coli |
C117A/A128A | kcat is 1.3fold lower than wild-type value, KM is 1.5fold higher than wild-type value | Escherichia coli |
C128A | kcat is 1.5fold higher than wild-type value, KM is 1.5fold higher than wild-type value | Escherichia coli |
additional information | substitution of cysteine for amino acids with nonnucleophilic side chains does not significantly alter the catalytic activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000108 | - |
tRNA uridine32 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme | Escherichia coli | |
0.000157 | - |
tRNA uridine32 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A/C128A | Escherichia coli | |
0.00016 | - |
tRNA uridine32 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C128A | Escherichia coli | |
0.00042 | - |
tRNA uridine32 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AA37 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
tRNA uridine32 = tRNA pseudouridine32 | RluA does not require thiol(ate) groups to effect catalysis | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA uridine32 | substrate: Escherichia coli tRNAPhe | Escherichia coli | tRNA pseudouridine32 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RluA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
tRNA uridine32 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A | Escherichia coli | |
0.074 | - |
tRNA uridine32 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A/C128A | Escherichia coli | |
0.099 | - |
tRNA uridine32 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme | Escherichia coli | |
0.15 | - |
tRNA uridine32 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C128A | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
166.7 | - |
tRNA uridine32 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A | Escherichia coli | |
471.3 | - |
tRNA uridine32 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C117A/C128A | Escherichia coli | |
916.7 | - |
tRNA uridine32 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme | Escherichia coli | |
937.5 | - |
tRNA uridine32 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C128A | Escherichia coli |