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Literature summary for 5.4.99.27 extracted from

  • Urban, A.; Behm-Ansmant, I.; Branlant, C.; Motorlin, Y.
    RNA sequence and two-dimensional structure features required for efficient substrate modification by the Saccharomyces cerevisiae RNA:PSI-synthase Pus7p (2009), J. Biol. Chem., 284, 5845-5858.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
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 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q08647
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-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine13 the multisite-specific enzyme Pus7p is able to modify uridine13 in several yeast tRNAs, uridine35 in the pre-tRNATyr, uridine35 in U2 small nuclear RNA, and uridine50 in 5S rRNA. Using site-directed mutagenesis, a set of RNA variants derived from the yeast tRNAAsp(GUC), pretRNA Tyr(G(pseudouridine)A), and U2 small nuclear RNA is designed and the ability to be modified by Pus7p in vitro is tested. Pseudouridine13 formation in tRNAAsp(GUC). Does not depend on correct tRNA three-dimensional structure but is increased by the presence of RNA helices Saccharomyces cerevisiae tRNA pseudouridine13
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Synonyms

Synonyms Comment Organism
Pus7p
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 Saccharomyces cerevisiae
RNA:pseudouridine-synthase Pus7p
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 Saccharomyces cerevisiae
RNA:PSI-synthase Pus7p
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 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Saccharomyces cerevisiae